DOPAMINE TRANSPORTER/MU OPIATE RECEPTOR--CELLULAR AND SUBCELLULAR LOCALIZATIONS

多巴胺转运蛋白（DAT）和μ阿片受体（莫尔）已被发现， 被确定为主要的大脑受体网站与 可卡因和吗啡的奖励和欣快特性。蜂窝和 亚细胞定位数据对于帮助阐明 这些重要的大脑分子的功能特性。在本财年， 大鼠脑内DAT和莫尔蛋白分布的研究 本文报告 对转运蛋白的超微结构研究显示， 大多数DAT和莫尔免疫反应性见于 细胞内和质膜定位不容易相关 与传统的突触特化。这些研究基本上 添加到详细的细胞定位的信息， 可卡因和吗啡的重要场所他们强调， 突触外多巴胺的清除可能提供了一个主要的 DAT的功能。

The dopamine transporter (DAT) and mu opiate receptor (MOR) have been identified as the principal brain receptor sites correlated with the rewarding and euphoric properties of cocaine and morphine. Cellular and subcellular localization data are important to help to elucidate the functional properties of these important brain molecules. In this FY, studies of the distribution of the DAT and MOR proteins in rat brains were reported. Ultrastructural studies of the transporter revealed that most of the DAT and MOR immunoreactivity was found in intracellular and plasma membrane localizations not readily associated with conventional synaptic specializations. These studies substantially add to information on the detailed cellular localizations of these important sites for cocaine and morphine reward. They underscore the likelihood that extrasynaptic removal of dopamine provides a principal function of DAT.