DOPAMINE TRANSPORTER/MU OPIATE RECEPTOR--CELLULAR AND SUBCELLULAR LOCALIZATIONS

多巴胺转运蛋白/MU阿片受体——细胞和亚细胞定位

• 批准号: 6161706
• 负责人: G R UHL
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161706
• 关键词: cocaine; dopamine transporter; dorsal horn; enkephalins; immunocytochemistry; laboratory rat; neuroanatomy; neuropharmacology; nucleus accumbens; opioid receptor; receptor binding; synapses

The dopamine transporter (DAT) and mu opiate receptor (MOR) have been identified as the principal brain receptor sites correlated with the rewarding and euphoric properties of cocaine and morphine. Cellular and subcellular localization data are important to help to elucidate the functional properties of these important brain molecules. In this FY, studies of the distribution of the DAT and MOR proteins in rat brains were reported. Ultrastructural studies of the transporter revealed that most of the DAT and MOR immunoreactivity was found in intracellular and plasma membrane localizations not readily associated with conventional synaptic specializations. These studies substantially add to information on the detailed cellular localizations of these important sites for cocaine and morphine reward. They underscore the likelihood that extrasynaptic removal of dopamine provides a principal function of DAT.

多巴胺转运体(DAT)和单位阿片受体(MOR) 被确定为主要的大脑感受器位置与 可卡因和吗啡的奖赏和愉悦特性。蜂窝和 亚细胞定位数据对于帮助阐明 这些重要的大脑分子的功能特性。在本财年， 大鼠脑内DAT和MOR蛋白分布的研究 都被报道过。对转运蛋白的超微结构研究揭示 大多数DAT和MOR免疫反应是在 细胞内和质膜的定位不容易关联 通过传统的突触专门化。这些研究实质上 添加有关这些组件的详细蜂窝定位的信息 可卡因和吗啡奖励的重要地点。它们强调了 突触外移除多巴胺的可能性提供了一个主要的 DAT的功能。