TUMOR SUPPRESSOR GENE AT 13Q21 IN PROSTATE CANCER

2021 年第 13 季度前列腺癌中的肿瘤抑制基因

• 批准号: 6191110
• 负责人: JIN-TANG DONG
• 金额: $ 24.12万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-18 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6191110
• 关键词: apoptosis; artificial chromosomes; athymic mouse; carcinogenesis; cell line; cell proliferation; clinical research; complementary DNA; gene deletion mutation; genetic polymorphism; homozygote; human subject; loss of heterozygosity; male; neoplasm /cancer genetics; polymerase chain reaction; prostate neoplasms; sequence tagged sites; single strand conformation polymorphism; tumor suppressor genes

DESCRIPTION (Adapted from investigator's abstract): Prostate cancer has a complex pathogenesis in which many men develop early stage 'benign' cancers, and a much smaller but substantial number develop aggressive cancers that metastasize and cause premature death. The PI has identified a region on chromosome 13q21 which appears to contain a gene whose alteration contributes to the aggressive behavior of prostate cancer. This region is subject to LOH and homozygous deletion in aggressive prostate cancers and cell lines. The current minimal region contains at least three genes which are expressed in normal prostate and low-grade tumors, but not aggressive cancers. The PI now proposes to define the smallest region of deletions that contains the 13q21 gene; to identify the 13q21 tumor suppressor gene (TSG) through expression and mutation analyses; to express the gene in cancer cell lines to examine effects on growth and metastasis; and examine in greater detail how often LOH and homozygous deletion affect the gene, the expression of the gene in prostate cancer, and clinical and pathologic correlations including prognostic significance.

描述（改编自研究者摘要）：前列腺癌具有 复杂的发病机制，其中许多男性发展为早期“良性”癌症， 还有少得多但相当多的人患上了侵袭性癌症， 转移并导致过早死亡PI已经确定了一个区域， 染色体13 q21似乎含有一个基因，其改变有助于 前列腺癌的侵袭行为。该区域易发生洛缺失 以及侵袭性前列腺癌和细胞系中的纯合缺失。的 当前最小区域包含至少三个基因， 正常前列腺和低度恶性肿瘤，但不是侵袭性癌症。现在的PI 建议定义包含13 q21的最小缺失区域 基因;通过表达鉴定13 q21肿瘤抑制基因（TSG）， 突变分析;在癌细胞系中表达基因以检查效果 以及更详细地检查洛缺失和 纯合性缺失影响该基因在前列腺中的表达 癌症，以及临床和病理相关性，包括预后 意义