SCREENING VESTIBULAR FUNCTION USING EVOKED POTENTIALS

使用诱发电位筛查前庭功能

• 批准号: 6176010
• 负责人: SHERRI M JONES
• 金额: $ 18.97万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-27 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176010
• 关键词: auditory stimulus; auditory threshold; balance; brain electrical activity; brain stem; computer program /software; computer system hardware; developmental neurobiology; diagnosis design /evaluation; diagnosis quality /standard; ear disorder diagnosis; evoked potentials; genetic strain; hearing; laboratory mouse; labyrinth; mutant; neurons; noninvasive diagnosis; posture; reflex; temporal bone; vestibular apparatus

There are no direct, noninvasive, physiological measures of vestibular function. This is in clear contrast to the wide variety of physiological measures available to directly assess function of the auditory system at all levels from the end organ to the cortex. Many of these auditory measures, most notably, auditory brainstem responses, have been used to screen for, or identify and characterize hearing loss in a large number of genetic mutants. These studies have provided important information about the genetics of hearing impairment. The proposed research will develop the techniques to assess the functional status of the vestibular end organs and eighth nerve. Meaurements will be direct, noninvasive and implemented by adapting established techniques for recording linear vestibular evoked potentials. Protocols will be suitable for screening and detailed functional assessment. Two specific aims will be addressed. First, stimulation and recording hardware and software will be developed. Peripheral vestibular and brainstem neural activity will be recorded using far-field evoked potential techniques. Adequate stimuli for activation of macular or ampullar neurons will be used to elicit responses. Stimuli will be applied to the cranium via a mechanical shaker/head mount system. Normal mice and genetic mutants with specific inner ear or central anomalies will be used to demonstrate test validity. Second, the effectiveness and efficiency of the measurement technique will be evaluated. Vestibular function will be surveyed based on two selection strategies. One strategy will measure mice that display behavioral signs of imbalance or vestibular dysfunction or have measurable hearing loss. The second strategy will measure random samples drawn from multiple genetic strains where vestibular dysfunction may be obscure or hidden . This research will produce a tool for the direct, noninvasive assessment of vestibular function and will generate a database quantifying vestibular function in relation to genetics. The knowledge gained will serve as a basis for future research ultimately leading to a better understanding of vestibular ontogeny, genetics of vestibular impairment and better diagnosis and treatment of dizziness in humans.

没有直接的、无创的、对前庭功能的生理测量。这与直接评估从末端器官到皮层各级听觉系统功能的各种生理测量方法形成鲜明对比。许多这些听觉测量，最值得注意的是，听觉脑干反应，已被用于筛选，或识别和描述大量基因突变的听力损失。这些研究提供了有关听力障碍遗传学的重要信息。本研究将发展评估前庭末梢器官和第八神经功能状态的技术。测量将是直接的，非侵入性的，并通过采用已建立的记录线性前庭诱发电位的技术来实现。方案将适用于筛选和详细的功能评估。将讨论两个具体目标。首先，将开发刺激和记录硬件和软件。外周前庭和脑干神经活动将使用远场诱发电位技术记录。适当的刺激，以激活黄斑或壶腹神经元将被用来引发反应。刺激将通过机械摇床/头戴系统应用于颅骨。将使用正常小鼠和具有特定内耳或中枢异常的基因突变来证明测试的有效性。其次，评估测量技术的有效性和效率。前庭功能将根据两种选择策略进行调查。一种方法是测量那些表现出行为失衡、前庭功能障碍或听力损失的老鼠。第二种策略将测量从多个基因株中抽取的随机样本，其中前庭功能障碍可能是模糊的或隐藏的。这项研究将为前庭功能的直接、无创评估提供一种工具，并将产生一个量化与遗传学有关的前庭功能的数据库。所获得的知识将作为未来研究的基础，最终导致更好地了解前庭个体发育，前庭损伤的遗传学以及更好地诊断和治疗人类头晕。