SCREENING VESTIBULAR FUNCTION USING EVOKED POTENTIALS

使用诱发电位筛查前庭功能

• 批准号: 6379553
• 负责人: SHERRI M JONES
• 金额: $ 10.58万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-27 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379553
• 关键词: auditory stimulus; auditory threshold; balance; brain electrical activity; brain stem; computer program /software; computer system hardware; developmental neurobiology; diagnosis design /evaluation; diagnosis quality /standard; ear disorder diagnosis; evoked potentials; genetic strain; hearing; laboratory mouse; labyrinth; mutant; neurons; noninvasive diagnosis; posture; reflex; temporal bone; vestibular apparatus

There are no direct, noninvasive, physiological measures of vestibular function. This is in clear contrast to the wide variety of physiological measures available to directly assess function of the auditory system at all levels from the end organ to the cortex. Many of these auditory measures, most notably, auditory brainstem responses, have been used to screen for, or identify and characterize hearing loss in a large number of genetic mutants. These studies have provided important information about the genetics of hearing impairment. The proposed research will develop the techniques to assess the functional status of the vestibular end organs and eighth nerve. Meaurements will be direct, noninvasive and implemented by adapting established techniques for recording linear vestibular evoked potentials. Protocols will be suitable for screening and detailed functional assessment. Two specific aims will be addressed. First, stimulation and recording hardware and software will be developed. Peripheral vestibular and brainstem neural activity will be recorded using far-field evoked potential techniques. Adequate stimuli for activation of macular or ampullar neurons will be used to elicit responses. Stimuli will be applied to the cranium via a mechanical shaker/head mount system. Normal mice and genetic mutants with specific inner ear or central anomalies will be used to demonstrate test validity. Second, the effectiveness and efficiency of the measurement technique will be evaluated. Vestibular function will be surveyed based on two selection strategies. One strategy will measure mice that display behavioral signs of imbalance or vestibular dysfunction or have measurable hearing loss. The second strategy will measure random samples drawn from multiple genetic strains where vestibular dysfunction may be obscure or hidden . This research will produce a tool for the direct, noninvasive assessment of vestibular function and will generate a database quantifying vestibular function in relation to genetics. The knowledge gained will serve as a basis for future research ultimately leading to a better understanding of vestibular ontogeny, genetics of vestibular impairment and better diagnosis and treatment of dizziness in humans.

前庭功能没有直接的、非侵入性的生理测量。 这与可用于直接评估从终末器官到皮层的所有水平的听觉系统功能的各种生理测量形成鲜明对比。 许多这些听觉措施，最值得注意的是，听觉脑干反应，已被用来筛选，或识别和表征听力损失的大量遗传突变体。 这些研究提供了有关听力障碍遗传学的重要信息。这项研究将发展评估前庭终器和第八神经功能状态的技术。测量将是直接的，非侵入性的，并通过调整已建立的记录线性前庭诱发电位的技术来实现。 方案将适用于筛选和详细的功能评估。 将讨论两个具体目标。 首先，将开发刺激和记录硬件和软件。 将使用远场诱发电位技术记录外周前庭和脑干神经活动。 激活黄斑或壶腹神经元的适当刺激将用于引起反应。 刺激将通过机械振动器/头部安装系统施加到颅骨。 正常小鼠和具有特定内耳或中枢异常的遗传突变体将用于证明试验有效性。第二，将评估衡量技术的有效性和效率。 前庭功能将根据两种选择策略进行调查。 一种策略是测量表现出不平衡或前庭功能障碍的行为迹象或具有可测量的听力损失的小鼠。 第二种策略将测量从多个遗传菌株中抽取的随机样本，其中前庭功能障碍可能是模糊或隐藏的。这项研究将产生一种直接，非侵入性评估前庭功能的工具，并将产生一个数据库，量化前庭功能与遗传学的关系。 所获得的知识将作为未来研究的基础，最终导致更好地了解前庭个体发育，前庭损伤的遗传学以及更好地诊断和治疗人类头晕。