BIOLOGIC BASES OF REFRACTORY PERIODONTAL DIS

描述(改编自调查人员摘要)：本报告的目标 建议是检验难治性疾病是独特的这一假设 从非顽固性牙周病中分离出来，并识别一个或多个 难治性受试者内明确的证候分析 微生物和寄主因素。具体目标是：1)确定 难治性研究对象中的主要细菌，包括未识别的和 使用16S rRNA基因序列的未栽培物种。分析16S rDNA 从斑块文库中克隆的基因。鉴定未开垦的口腔物种 并确定它们在牙周病中的作用。2)检验假设 患有顽固性疾病的受试者可以与受试者区分开来 基于微生物的可治疗牙周炎和牙周健康 组成。每组40名受试者的28个地点将被选为 对60个可栽培类群和120个未栽培类群的水平进行了分析 棋盘杂交试验。允许识别符合以下条件的物种 有助于区分顽固性和可治疗的牙周炎和 健康。3)检验难治性疾病受试者(S)可以 区别于那些有可治疗的疾病和健康的人 宿主因素，如基因类型、同种异型、血清IgG2水平和特异性 40种特定牙周细菌的抗体水平。4)确定更多信息 基于微生物的难治性组内的1个以上疾病综合征 和宿主标记。

DESCRIPTION (Adapted from investigator's Abstract): The objectives of this proposal are to examine the hypothesis that refractory disease is distinct from nonrefractory periodontal disease and to identify one or more well-defined syndromes within the refractory subjects based on analysis of microbial and host factors. The specific aims are: 1) identify the predominant bacteria in refractory subjects including unrecognized and uncultivated species using 16S rRNA gene sequences. Analyzing 16S rDNA genes cloned from plaque libraries. Identify the uncultivated oral species and determine their role in periodontal disease. 2) Test the hypothesis that subjects with refractory disease can be distinguished from subjects with treatable periodontitis and periodontal health based upon microbial composition. Twenty-eight sites in 40 subjects in each of 3 groups will be analyzed for levels of 60 cultivable taxa and 120 uncultivated taxa using checkerboard hybridization assays. Allow identification of species that are useful for distinguishing between refractory and treatable periodontitis and health. 3) Test the hypothesis that subjects with refractory disease(s) can be distinguished from those with treatable disease and health based upon host factors such as, genotypes, allotypes, serum IgG2 levels and specific antibodies levels to 40 specific periodontal bacteria. 4) Identify more than 1 disease syndromes within the refractory group based upon microbial and host markers.