BIOLOGIC BASES OF REFRACTORY PERIODONTAL DIS

描述（改编自研究者摘要）：本研究的目的 我们的建议是检查难治性疾病是不同的假设， 从非难治性牙周病，并确定一个或多个 根据对难治性受试者的分析， 微生物和宿主因素。 具体目标是：1）确定 难治性受试者中的优势细菌，包括未识别的和 未栽培的物种使用16S rRNA基因序列。 16S rDNA分析 从噬菌斑文库克隆的基因。 识别未栽培的口腔物种 并确定它们在牙周病中的作用。 2)检验这一假设 患有难治性疾病的受试者可以与 可治疗的牙周炎和牙周健康基于微生物 混合物. 将在3组中每组40例受试者的28个研究中心 分析了60个可栽培类群和120个未栽培类群的水平， 棋盘杂交测定。 允许识别 用于区分难治性和可治疗的牙周炎， 健康 3)检验难治性疾病受试者可以 区别于那些可治疗的疾病和健康的基础上， 宿主因素，如基因型、同种异型、血清IgG 2水平和特异性 40种特定牙周细菌的抗体水平。 4)识别更多 基于微生物的难治性组中有超过1种疾病综合征 和宿主标记。

DESCRIPTION (Adapted from investigator's Abstract): The objectives of this proposal are to examine the hypothesis that refractory disease is distinct from nonrefractory periodontal disease and to identify one or more well-defined syndromes within the refractory subjects based on analysis of microbial and host factors. The specific aims are: 1) identify the predominant bacteria in refractory subjects including unrecognized and uncultivated species using 16S rRNA gene sequences. Analyzing 16S rDNA genes cloned from plaque libraries. Identify the uncultivated oral species and determine their role in periodontal disease. 2) Test the hypothesis that subjects with refractory disease can be distinguished from subjects with treatable periodontitis and periodontal health based upon microbial composition. Twenty-eight sites in 40 subjects in each of 3 groups will be analyzed for levels of 60 cultivable taxa and 120 uncultivated taxa using checkerboard hybridization assays. Allow identification of species that are useful for distinguishing between refractory and treatable periodontitis and health. 3) Test the hypothesis that subjects with refractory disease(s) can be distinguished from those with treatable disease and health based upon host factors such as, genotypes, allotypes, serum IgG2 levels and specific antibodies levels to 40 specific periodontal bacteria. 4) Identify more than 1 disease syndromes within the refractory group based upon microbial and host markers.