CORE--SIMIAN AND RODENT IMMUNOLOGY FACILITY

该核心免疫学实验室的中和抗体组成部分将为该计划的所有三个项目提供支持。具体来说，我们将评估针对SIV、HIV-1和猿人免疫缺陷病毒（SHIV）的同源和异源毒株的中和抗体的大小、广度和持续时间。试验将酌情在人CD4+ T细胞系或外周血单核细胞（PBMC）中进行，并将包括T细胞系适应型（TCLA）变体和HIV- 1的多个初级分离株。这些评估将允许详细评估分别由项目1和项目2中描述的修饰重组VRP疫苗载体和修饰形式的Env产生的中和抗体。它们还将被设计用于评估中和抗体是否与项目3中指定的猕猴实验性SIV攻击免疫相关。在项目1、2和3中引起的细胞免疫反应将在核心的细胞免疫学部分进行评估。将测量CTL和细胞因子合成使用细胞内测定。此外，将使用四聚体染色，并为特定动物生产特定的四聚体。

The neutralizing antibody component of this core imrnunology laboratory will provide support for all three projects of this program. Specifically, we will assess the magnitude, breadth and duration of neutralizing antibodies against homologous and heterologous strains of SIV, HIV-1 and simian-human immunodeficiency virus (SHIV). Assays will be performed in either human CD4+ T cell lines or peripheral blood mononuclear cells (PBMC) as appropriate, and will include a T cell line adapted (TCLA) variant and multiple primary isolates of HIV- l. These assessments will permit a detailed evaluation of neutralizing antibodies generated by modified recombinant VRP vaccine vectors and modified forms of Env as described in Projects I and 2, respectively. They will also be designed to evaluate neutralizing antibodies as a correlate of immunity against experimental SIV challenge in macaques as specified in Project 3. Cellular immune responses elicited in Projects I, 2 and 3, will be assessed in the cellular immunology component of the core. CTL will be measured as will cytokine synthesis using intracellular assays. Further, tetramer staining will used and specific tetramers produced for particular animals.