Route of Infection Shapes Immune Responses to Francisella

这一建议的重点是最早的相互作用的F。土拉菌病是土拉菌病的病原体， 主持人我们的假设是，肺中特定细胞的感染导致不同的宿主模式 产生的衍生免疫调节分子，其塑造宿主先天性和适应性免疫应答 有益于病原体在这项研究中，我们将确定肺部和皮肤感染中感染的早期细胞。我们 将确定感染后最早期细胞中产生的分子， 荧光激活细胞分选和标记细菌。通过细胞纯化和体外感染， 了解如何影响随后的免疫反应。然后我们将确定F。土拉热基因 负责，以及他们与宿主的互动。最后，我们将确定免疫机制 调变

This proposal focuses on the earliest interactions of the F. tularensis the causative agent of tularemia with the host. Our hypothesis is that infection of particular cells in the lung results in a different pattern of host derived immunomodulatory molecules produced that shape the host innate and adaptive immune responses to benefit the pathogen. In this study we will identify the early cells infected in lung and skin infections. We will determine the molecules produced in the earliest cells following infection using a combination of fluorescence activated cell sorting and marked bacteria. By cell purification and in vitro infection we will learn how the impact the subsequent immune response. We will then determine the F. tularensis genes responsible, and their interactions with the host. Finally, we will determine mechanism for immune modulation.