Route of Infection Shapes Immune Responses to Francisella

这项建议集中在图拉热症的病原体图拉氏杆菌与图拉热症的早期相互作用。 主持人。我们的假设是，肺部特定细胞的感染会导致不同的宿主模式 衍生的免疫调节分子产生塑造宿主先天和获得性免疫反应的分子 使病原体受益。在这项研究中，我们将鉴定肺部和皮肤感染的早期细胞。我们 将确定感染后最早的细胞中产生的分子 荧光激活细胞分选和标记细菌。通过细胞纯化和体外感染，我们将 了解如何影响后续的免疫反应。然后我们将确定图拉氏F菌的基因 负责，以及他们与主持人的互动。最后，我们将确定免疫机制 调制。

This proposal focuses on the earliest interactions of the F. tularensis the causative agent of tularemia with the host. Our hypothesis is that infection of particular cells in the lung results in a different pattern of host derived immunomodulatory molecules produced that shape the host innate and adaptive immune responses to benefit the pathogen. In this study we will identify the early cells infected in lung and skin infections. We will determine the molecules produced in the earliest cells following infection using a combination of fluorescence activated cell sorting and marked bacteria. By cell purification and in vitro infection we will learn how the impact the subsequent immune response. We will then determine the F. tularensis genes responsible, and their interactions with the host. Finally, we will determine mechanism for immune modulation.