PROTECTIVE EPITOPES IN TICK IMMUNITY
蜱免疫中的保护性表位
基本信息
- 批准号:6169698
- 负责人:
- 金额:$ 29.37万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-08-01 至 2002-07-31
- 项目状态:已结题
- 来源:
- 关键词:Borrelia Ehrlichia Ixodes Lyme disease antigen antibody reaction arthropod borne communicable disease bacterial antigens bactericidal immunity borreliosis communicable disease transmission ehrlichiosis genetic library guinea pigs high performance liquid chromatography host organism interaction immunoaffinity chromatography immunofluorescence technique laboratory mouse molecular cloning nucleic acid sequence passive immunization polymerase chain reaction protein purification salivary glands surface antigens western blottings
项目摘要
The proposed project is to identify the molecular basis of the antigens
involved in tick immunity and study its effects upon the transmission of
Lyme borreliosis and Ehrlichiosis.
Specifically a model of tick protective immunity has been established in
guinea pigs. Antibodies already shown to be capable of passively
protecting recipients will be purified using chemical and immunoabsorbent
methods. Tick salivary antigens will be obtained for Ixodes scapularis;
these antigens will be purified by chemical and immunological means using
the purified protective antibodies described above. Such antigens will be
used to actively produce tick immunity in the model.
With purified antibodies obtained from tick-sensitized animals, cDNA
libraries already prepared from salivary glands will be screened. Such
libraries, prepared from stimulated tick salivary glands, will be used to
clone genes encoding protective antigens. Several putative clones have
already been identified.
Recombinant antigens will be expressed, purified, and characterized before
use as immunogens to immunize rice and guinea pigs to determine their
potential for induction of anti-tick immunity.
Using immune guinea pigs, the effect of anti-tick immunity on the ability
of ticks infected with either Borrelia burgdorferi or Ehrlichia canis to
transmit disease to tick-immune hosts will be determined. The kinetics of
transmission from infecting tick to guinea pig host will be the basis of
studies on the mechanism of how anti-tick immunity affects pathogen
transmission.
拟议的项目是确定抗原的分子基础
参与蜱免疫,并研究其对传播的影响,
莱姆病和埃里希体病。
具体地说,已建立了蜱保护性免疫模型,
豚鼠抗体已经显示出能够被动地
保护受体将使用化学和免疫吸附剂进行纯化,
方法.将获得肩胛硬蜱的蜱唾液抗原;
这些抗原将通过化学和免疫学方法纯化,
上述纯化的保护性抗体。这些抗原将是
用于在模型中主动产生蜱免疫。
用从蜱致敏动物中获得的纯化抗体,
筛选已经从唾液腺制备的文库。等
从刺激的蜱唾液腺制备的文库将用于
克隆保护性抗原基因。几个假定的克隆体
已经被确认了。
重组抗原将在表达、纯化和表征之前进行
作为免疫原,免疫水稻和豚鼠,
诱导抗蜱免疫的潜力。
采用免疫豚鼠,观察抗蜱免疫对豚鼠抗蜱能力的影响,
感染伯氏疏螺旋体或犬埃立克体的蜱,
将疾病传播给蜱免疫宿主。的动力学
从感染蜱到豚鼠宿主的传播将是
抗蜱免疫对病原体作用机制的研究
传输
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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FRED S KANTOR其他文献
FRED S KANTOR的其他文献
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{{ truncateString('FRED S KANTOR', 18)}}的其他基金
PROTECTIVE MECHANISMS IN THE INTERFACE BETWEEN SPIROCHETE, TICK AND HOST
螺旋体、蜱虫和宿主之间界面的保护机制
- 批准号:
6099482 - 财政年份:1999
- 资助金额:
$ 29.37万 - 项目类别:
PROTECTIVE MECHANISMS IN THE INTERFACE BETWEEN SPIROCHETE, TICK AND HOST
螺旋体、蜱虫和宿主之间界面的保护机制
- 批准号:
6268036 - 财政年份:1998
- 资助金额:
$ 29.37万 - 项目类别:
PROTECTIVE MECHANISMS IN THE INTERFACE BETWEEN SPIROCHETE, TICK AND HOST
螺旋体、蜱虫和宿主之间界面的保护机制
- 批准号:
6234976 - 财政年份:1997
- 资助金额:
$ 29.37万 - 项目类别:
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