PROTECTIVE MECHANISMS IN THE INTERFACE BETWEEN SPIROCHETE, TICK AND HOST

螺旋体、蜱虫和宿主之间界面的保护机制

• 批准号: 6099482
• 负责人: FRED S KANTOR
• 金额: $ 17.16万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099482
• 关键词: Borrelia; Ixodes; Lyme disease; SDS polyacrylamide gel electrophoresis; active immunization; antigens; bactericidal immunity; communicable disease transmission; disease /disorder model; disease vectors; genetic library; guinea pigs; host organism interaction; humoral immunity; immunity; immunoglobulin G; laboratory mouse; laboratory rabbit; molecular cloning; monoclonal antibody; passive immunization; protein purification; salivary glands; surface antigens; tissue /cell culture; western blottings

Acquired resistance to ticks was reported in the late l93Os. These findings have been confirmed and extended to guinea pigs and rabbits using several species of ticks by several investigators including ourselves. The proposed project is to identify the molecular basis of the antigens involved in tick immunity and study its effect on the antigens of the B. burgdorferi carried by the ticks and upon the transmission of Lyme borreliosis. Specifically models of tick protective immunity will be established in guinea pigs and in rabbits. Antibodies shown to be capable of passively protecting recipients will be purified using chemical and immunoabsorbent methods. Antigen specificity of lymphocytes from tick protected animals and from clones will be characterized. Tick Salivary and midgut antigens will be obtained from the appropriate organs of Ixodes scapularis and from supernatants and homogenates of tissue cultures; these antigens will be purified by chemical and immunological means. Such antigens will be used to produce tick immunity in the models chosen. With polyclonal and monoclonal antibodies obtained from tick-or purified antigen-sensitized animals DNA libraries will be screened. Such libraries, prepared from stimulated tick salivary glands and midguts, will be used to clone genes encoding protective antigens. Using immune guinea pigs and mice the effect of anti-tick immunity on the surface antigens of tick-borne Borrelia burgdorferi will be studied and the ability of infected ticks to transmit disease to tick-immune host determined. The potential synergistic effects of dual anti-tick and anti- Osp A immunity on the ability of infected ticks to transmit Lyme borreliosis will be investigated in the mouse model.

对扁虱的获得性抗药性是在1930年末报告的。这些 这一发现已得到证实，并推广到豚鼠和兔子身上 包括我们在内的几名研究人员发现了几种扁虱。 拟议的项目是确定抗原的分子基础。 并研究其对乙型流感病毒抗原的影响。 由扁虱携带的伯氏杆菌和莱姆病的传播 疏螺旋体病。 具体地说，将在#年建立扁虱保护性免疫模型。 在豚鼠和兔子体内。抗体被证明能够被动地 保护接受者将使用化学和免疫吸收剂进行净化 方法：研究方法。壁虱保护性动物淋巴细胞的抗原特异性 而来自克隆人的将被描述为。 扁虱唾液和中肠抗原将从适当的 肩部硬蜱的器官及其培养上清液和匀浆 组织培养；这些抗原将通过化学和 免疫学手段。这种抗原将被用来产生扁虱免疫力。 在所选的型号中。 与从扁虱或提纯的蚊子中获得的多克隆和单克隆抗体 将对抗原致敏的动物DNA文库进行筛选。是这样的 由受刺激的扁虱唾液腺和中肠准备的图书馆将 用于克隆编码保护性抗原的基因。 利用免疫豚鼠和小鼠，观察抗蚤免疫对小鼠免疫功能的影响。 将研究由壁虱传播的伯氏疏螺旋体表面抗原并 被感染的扁虱将疾病传播给扁虱免疫宿主的能力 下定决心。二抗滴虫和抗滴虫的潜在协同作用 OSP A对受感染的硬蜱传播莱姆病能力的免疫 将在小鼠模型中研究疏螺旋体病。