SIV CORECEPTOR USAGE DETERMINES CD4+T CELL DEPLETION

描述：这些研究的目的是使用一种不寻常的SIVmac239 变异体（EvT3），以确定CXCR4共受体的使用如何影响 SIV感染猕猴的CD4+ T细胞下降率。 第一 具体目的是鉴定EvT3在体外使用什么共受体。 第二个是确定共受体使用对于 SIV感染CD4+ T细胞亚群。 在第三个具体目标中， gp120糖蛋白中影响CXCR4使用的遗传决定因素 将研究体外和体内CD4耗竭效率。 具体目标4是确定CD4+ T细胞活化的意义 共同受体表达和致病作用。

DESCRIPTION: The goal of these studies is to use an unusual SIVmac239 variant (EvT3) to determine how useage of the CXCR4 co-receptor impacts on the rate of CD4+ T-cell decline in SIV-infected macaques. The first specific aim is to identify what co-receptors are used by EvT3 in vitro. The second is to determine the significance of co-receptor useage for the infection of CD4+ T-cell subsets by SIV. In the third specific aim, the genetic determinants in the gp120 glycoprotein that influence CXCR4 useage in vitro and CD4 depletion efficiency in vivo will be investigated. Specific aim 4 is to determine the significance of CD4+ T-cell activation for co-receptor expression and pathogenic effects.