SIV CORECEPTOR USAGE DETERMINES CD4+T CELL DEPLETION

描述：这些研究的目标是使用一种不寻常的SIVmac239 突变体(EvT3)，以确定CXCR4共受体的使用如何影响 SIV感染猕猴的CD4+T细胞下降率。第一 具体目的是确定EvT3在体外使用的是什么辅助受体。 第二个是确定共同受体的使用对 SIV感染对CD4+T细胞亚群的影响在第三个具体目标中， Gp120糖蛋白中影响CXCR4利用的遗传决定因素 体外和体内的CD4耗竭效率将被研究。 具体目的4是确定CD4+T细胞活化的意义 用于共受体的表达和致病作用。

DESCRIPTION: The goal of these studies is to use an unusual SIVmac239 variant (EvT3) to determine how useage of the CXCR4 co-receptor impacts on the rate of CD4+ T-cell decline in SIV-infected macaques. The first specific aim is to identify what co-receptors are used by EvT3 in vitro. The second is to determine the significance of co-receptor useage for the infection of CD4+ T-cell subsets by SIV. In the third specific aim, the genetic determinants in the gp120 glycoprotein that influence CXCR4 useage in vitro and CD4 depletion efficiency in vivo will be investigated. Specific aim 4 is to determine the significance of CD4+ T-cell activation for co-receptor expression and pathogenic effects.