REGULATION OF TRANSCRIPTIONAL ELONGATION BY HIV1 TAT
HIV1 TAT 对转录延伸的调节
基本信息
- 批准号:6170603
- 负责人:
- 金额:$ 23.51万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-08-01 至 2002-07-31
- 项目状态:已结题
- 来源:
- 关键词:DNA directed RNA polymerase HeLa cells RNA binding protein RNase protection assay cofactor enzyme activity gel electrophoresis gel mobility shift assay gene expression gene induction /repression genetic mapping genetic promoter element human immunodeficiency virus 1 immunoprecipitation laboratory mouse laboratory rabbit molecular cloning nucleic acid hybridization phosphorylation polymerase chain reaction protein kinase protein structure function regulatory gene transcription factor virus protein western blottings
项目摘要
Control of transcriptional elongation has been recognized as an
important step in gene regulation, but mechanisms regulating the
efficiency of elongation by RNA polymerase II have not been
extensively studied. The goal of the proposed research is to
elucidate mechanisms regulating elongation using HIV-l Tat as a
model system. Tat stimulates the efficiency of elongation by
recognizing the trans-acting-response (TAR) RNA element located
at the 5' end of the nascent viral transcript. Tat trans-activation
requires specific cellular cofactors. The applicant has recently
isolated and cloned a cellular cofactor, Tat-SFI, which is
specifically required for Tat activation of elongation and is a
substrate of an associated linase. Tat-SFI is distantly related to
EWS and FUS/TLS, which are members of a novel class of
putative transcription factors with RNA recognition motifs and are
frequently associated with sarcomas. Preliminary studies suggest
that Tat may stimulate the efficiency of elongation by recruiting a
preformed complex containing Tat-SFI and its kinase to the HIV
promoter and by enhancing the effect of an elongation factor,
Elongin (Sill). This proposal seeks to further dissect the function
of Tat-SFI in Tat activation through analyzing the sequence
specificity of the various interactions among Tat, Tat-SFI, the Tat-
SFI kinase, and TAR, and through mapping the and amino acid
residues critical for Tat-SFI activity. One important objective is to
assess the intriguing functional significance of Tat-SFI
phosphorylation in Tat activation and to isolate, clone, and
characterize its kinase. A significant effort will also be devoted to
the investigation of the mechanisms by which Tat and Tat SFI
stimulate the efficiency of elongation and the identification of
components of a Tat-stimulated elongating polymerase complex.
Finally, the relationship between Tat and cellular elongation
factors such as Elongin and TFIIS in activation of polymerase
processivity will be studied. Further analysis of Tat activation of
the efficiency of elongation and the role of the cellular cofactors
in this process will likely reveal general mechanisms of gene
regulation at the stage of elongation.
控制转录伸长已被认为是一种有效的方法
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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QIANG ZHOU其他文献
QIANG ZHOU的其他文献
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{{ truncateString('QIANG ZHOU', 18)}}的其他基金
Tat cofactors and control of HIV-1 latency
Tat辅助因子和HIV-1潜伏期的控制
- 批准号:
8434937 - 财政年份:2011
- 资助金额:
$ 23.51万 - 项目类别:
Tat cofactors and control of HIV-1 latency
Tat辅助因子和HIV-1潜伏期的控制
- 批准号:
8139592 - 财政年份:2011
- 资助金额:
$ 23.51万 - 项目类别:
Tat cofactors and control of HIV-1 latency
Tat辅助因子和HIV-1潜伏期的控制
- 批准号:
8236887 - 财政年份:2011
- 资助金额:
$ 23.51万 - 项目类别:
Tat cofactors and control of HIV-1 latency
Tat辅助因子和HIV-1潜伏期的控制
- 批准号:
8811092 - 财政年份:2011
- 资助金额:
$ 23.51万 - 项目类别:
Input competition and synaptic modification during developmental remodeling
发育重塑过程中的输入竞争和突触修饰
- 批准号:
7568566 - 财政年份:2009
- 资助金额:
$ 23.51万 - 项目类别:
Regulation of Transcriptional Elongation by HIV-1 Tat
HIV-1 Tat 对转录延伸的调节
- 批准号:
7925117 - 财政年份:2009
- 资助金额:
$ 23.51万 - 项目类别:
Activity-dependent Plasticity of Retinotectal Synapses
视网膜顶盖突触的活动依赖性可塑性
- 批准号:
6525067 - 财政年份:2002
- 资助金额:
$ 23.51万 - 项目类别:
Activity-dependent Plasticity of Retinotectal Synapses
视网膜顶盖突触的活动依赖性可塑性
- 批准号:
6784202 - 财政年份:2002
- 资助金额:
$ 23.51万 - 项目类别:
Activity-dependent Plasticity of Retinotectal Synapses
视网膜顶盖突触的活动依赖性可塑性
- 批准号:
6405058 - 财政年份:2001
- 资助金额:
$ 23.51万 - 项目类别:
Regulation of Transcriptional Elongation by HIV-1 TAT
HIV-1 TAT 对转录延伸的调节
- 批准号:
7190551 - 财政年份:1997
- 资助金额:
$ 23.51万 - 项目类别:
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