THE ROLE OF LYT1 PROTEIN IN TRYPANOSOMA CRUZI

描述（改编自申请人的摘要）：这项工作的目标 分析LYT 1 p蛋白在T. cruzi发展和 发病机制这种蛋白质首先在安东尼奥·冈萨雷斯的实验室中被发现， （西班牙），他通过用针对该蛋白的抗体筛选cDNA文库发现了该蛋白。 哺乳动物补体C9成分。LYT 1是一个单拷贝基因， 艾林湾克氏上鞭毛体。然而，LYT 1缺陷型寄生虫 三种表型：1）它们是感染缺陷型; 2）它们表现出加速的 体外发育;和3）它们具有降低的溶血活性。有 该项目的两个主要目标：1）了解LYT 1 p如何参与 导致人类感染的生物学过程; 2）使用LYT 1 p 缺陷型寄生虫，以鉴定寄生虫中涉及的阶段特异性基因 发展

DESCRIPTION (Adapted from the Applicant's Abstract): The objective of this work is to dissect the role of protein LYT1p in T. cruzi development and pathogenesis. This protein was first identified in the lab of Antonio Gonzalez (Spain) who found it by screening a cDNA library with antibody directed to the mammalian C9 component of complement. LYT1 is a single copy gene that is dispensable in T. cruzi epimastigotes. However, LYT1 deficient parasites have three phenotypes: 1) they are infection deficient; 2) they exhibit accelerated in vitro development; and 3) they have diminished hemolytic activity. There are two main goals in this project: 1) to understand how the LYT1p is involved in the biological processes leading to human infection; and 2) to use LYT1p deficient parasites to identify stage specific genes involved in parasite development.