THE ROLE OF LYT1 PROTEIN IN TRYPANOSOMA CRUZI

描述(改编自申请人的摘要)：本工作的目标 是剖析蛋白质LYT1p在克氏毛滴虫发育和发育中的作用 发病机制。这种蛋白质最初是在安东尼奥·冈萨雷斯的实验室中发现的。 (西班牙)，他通过筛选带有针对该基因的抗体的cDNA库发现了它。 哺乳动物补体的C9成分。LYT1是一种单拷贝基因， 在克氏锥虫上鞭毛虫中是可有可无的。然而，LYT1缺陷的寄生虫有 三种表型：1)缺乏感染；2)表现为加速 在体外发育；以及3)它们具有降低的溶血活性。确实有 本项目的两个主要目标：1)了解LYT1p如何参与 导致人类感染的生物学过程；和2)使用LYT1p 利用缺陷寄生虫鉴定与寄生虫相关的阶段特异性基因 发展。

DESCRIPTION (Adapted from the Applicant's Abstract): The objective of this work is to dissect the role of protein LYT1p in T. cruzi development and pathogenesis. This protein was first identified in the lab of Antonio Gonzalez (Spain) who found it by screening a cDNA library with antibody directed to the mammalian C9 component of complement. LYT1 is a single copy gene that is dispensable in T. cruzi epimastigotes. However, LYT1 deficient parasites have three phenotypes: 1) they are infection deficient; 2) they exhibit accelerated in vitro development; and 3) they have diminished hemolytic activity. There are two main goals in this project: 1) to understand how the LYT1p is involved in the biological processes leading to human infection; and 2) to use LYT1p deficient parasites to identify stage specific genes involved in parasite development.