ICER AND NORMAL AND NEOPLASTIC CELL GROWTH

描述(改编自申请人的摘要)：这些数据支持 哺乳动物中ICER作为抑癌基因发挥作用的工作假说 细胞，因为它的表达减慢了无法控制的细胞生长 是恶性细胞的特征。为了检验这一假设， 提出了以下具体目标：1)进一步明确角色特征 ICERIIGamma在AtT20细胞周期中的作用，2)确定 ICERIIGamma在细胞生长、增殖和肿瘤形成过程中的作用 以及3)确定ICERIIGamma活动是否受 磷酸化。这些目标将通过评估效果来实现 在特定的哺乳动物细胞系统中过表达ICERIIGamma。 完成这些目标将使我们能够更全面地了解 CAMP途径和CREM基因在细胞生长中的作用 分化和肿瘤发生。因此，这些结果将具有 对了解正常和非小细胞肺癌增殖的重大影响 肿瘤细胞。

DESCRIPTION (Adapted from the applicant's abstract): These data support the working hypothesis that ICER functions as an antioncogene in mammalian cells, since its expression decelerates the uncontrollable cell growth which is characteristic of malignant cells. To test this hypothesis, the following specific aims are proposed: 1) to further characterize the role of ICERIIgamma in the AtT20 cell cycle, 2) to determine the role of ICERIIgamma in cell growth, proliferation and the process of oncogenesis, and 3) to determine whether ICERIIgamma activity is regulated by phosphorylation. These aims will be achieved by assessment of the effects of overexpression of ICERIIgamma in specific mammalian cell systems. Completion of these aims will allow a more comprehensive understanding of the role played by the cAMP pathway and the CREM gene in cell growth, differentiation and tumorigenesis. These results will therefore have a significant impact upon understanding of proliferation of normal and neoplastic cells.