TEMPORAL CODING IN OLFACTORY BULB CIRCUITS

嗅觉灯泡电路中的时间编码

• 批准号: 6176086
• 负责人: KATHLEEN M DORRIES
• 金额: $ 5.74万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176086
• 关键词: GABA receptor; NMDA receptors; Urodela; brain electrical activity; dyes; electrostimulus; excitatory aminoacid; glutamate receptor; inhibitor /antagonist; membrane potentials; neuropharmacology; odors; olfactory lobe; olfactory stimulus

The immediate goal of this project is to understand the relationship between temporal spiking patterns in neurons in the olfactory bulb and odorant coding. The long term goal is to relate these finding to information coding in other regions of the brain. The hypothesis to be examined here is that spikes occurring at different times relative to a stimulus pulse are generated by different circuit components and thus carry different information about the stimulus. These experiments are designed to examine how vertebrate olfactory bulb circuits relate to spike patterns of the output mitral and tufted cells (MTs). Two questions will be addressed: 1)Using receptor antagonists as tools, how do NMDA and non-NMDA glutamate receptor inputs contribute differentially to odorant-elicited MT spiking patterns? The hypothesis is that non- NMDA input contributes more than NMDA input to short latency spiking, and thus has a larger effect on burst latency; 2) Do GABAergic inputs contribute differentially to both early and late periods of suppression in MT odorant responses? GABA and a GABA receptor antagonist will be applied during extracellular recording to test the hypothesis that GABAergic inputs contribute to both MT spiking latency and to suppression following spiking. Although, as described below, there have been a number of studies in which pharmacological tools have been used to dissect responses elicited by electrical stimulation, few have used these tools during odor stimulation, and in the vertebrate none have related these response components to the behavioral capabilities of the animal. The projects proposed here will provide new information on this important aspect of the odorant encoding process, and further our knowledge of information processing in sensory systems, in learning and memory, and in the coding and categorization of information during decision making processes. Studies such as these will be critical in understanding brain/behavior relationships.

该项目的直接目标是了解这种关系 嗅球神经元的时间尖峰模式之间 气味编码。 长期目标是将这些发现与 大脑其他区域的信息编码。 假设为 这里检查的是相对于不同时间发生的尖峰 刺激脉冲是由不同的电路元件产生的，因此 携带有关刺激的不同信息。 这些实验是 旨在检查脊椎动物嗅球电路如何与 输出二尖瓣和簇状细胞 (MT) 的尖峰模式。二 将解决以下问题：1）使用受体拮抗剂作为工具，如何 NMDA 和非 NMDA 谷氨酸受体输入的贡献是否不同 气味引起的 MT 尖峰模式？ 假设是非 NMDA 输入对短延迟尖峰的贡献大于 NMDA 输入， 从而对突发延迟有较大影响； 2) GABA能输入 对早期和晚期抑制的贡献不同 在 MT 气味反应中？ GABA 和 GABA 受体拮抗剂将 在细胞外记录期间应用来检验以下假设 GABAergic 输入会导致 MT 尖峰延迟和 尖峰后的抑制。 尽管如下所述，有 许多研究都使用了药理学工具 为了剖析电刺激引起的反应，很少有人使用 这些工具在气味刺激过程中发挥作用，而脊椎动物则没有 将这些反应成分与行为能力联系起来 动物。 这里提出的项目将提供这方面的新信息 气味编码过程的重要方面，并进一步我们 感觉系统、学习和信息处理的知识 记忆，以及信息的编码和分类 决策过程。 诸如此类的研究对于 了解大脑/行为关系。