CORE--ANTIBODY/RADIOIMMUNOASSAY

核心--抗体/放射免疫分析

• 批准号: 6316589
• 负责人: JOHN H WALSH
• 金额: $ 14.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316589
• 关键词: biomedical facility; gastrointestinal system; immunocytochemistry; immunofluorescence technique; immunologic assay /test; monoclonal antibody; radioimmunoassay; radiotracer; technology /technique development; tissue /cell culture

DESCRIPTION (Adapted from the application) The objectives of this core are to develop polyclonal and monoclonal antibodies useful for radioimmunoassay, for immunohistochemistry, and for immunoneutralization and to assist center members in the use of these antibodies. A second objective is to prepare radiolabeled and fluorescent peptides for use in radioimmunoassays, receptor binding, and receptor internalization studies. The core produces the antibody, develops the required assays, and provides the kits that are used by members at the center according to their own experiments. The core also provides teaching services for techniques and procedures including Western blot analysis, radio-iodination, ELISA, antibody production, antibody purification and Fc fragment production, RIA procedures, and fluorescence conjugations. These services will be used by CURE investigators, technicians, and postdoctoral fellows. In addition, the core will perform non-routine RIAs upon request. Antisera and monoclonal antibodies produced by the core are tabulated and are made available to center investigators. These available antibodies are listed on the CURE Web page and are made available to investigators outside the center. The space for this core consists of twin 500 square feet laboratories in the UCLA Division of Digestive Diseases adjacent to the office of Dr. Walsh. The laboratory is fully equipped for its current functions. Previously produced antibodies are readily distributed upon request after approval of the Director. Request for preparation of new antibodies requires consultation with the Director to determine the feasibility of the project and the most practical approach. A large number of center investigators, as well as scientists worldwide, have been provided core services, and many papers have resulted from interactions with this core. Between 1993 and June 1998, eighty-seven papers from the center have resulted from interactions with this core. During the period, there are 44 additional papers published by investigators who were not members of the CURE but who utilized the core services.

描述（根据应用程序改编）该核心的目标是 开发多克隆和单克隆抗体对放射免疫测定有用，用于 免疫组织化学和免疫化学化并协助中心成员 在使用这些抗体中。第二个目标是准备放射标记的 和荧光肽用于放射免疫测定，受体结合和 受体内在化研究。核心产生抗体，发展 必需的测定法，并提供中心成员使用的套件 根据他们自己的实验。核心还提供教学服务 对于包括Western印迹分析在内的技术和程序， 射线碘，ELISA，抗体产生，抗体纯化和FC 碎片产生，RIA程序和荧光结合。这些 治疗研究人员，技术人员和博士后将使用服务 研究员。此外，核心将根据要求执行非列表RIAS。 核心产生的抗血清和单克隆抗体被列举，并且是 可用于中心调查员。这些可用抗体已列出 在Cure网页上，可供调查人员使用 中心。 该核心的空间由Twin 500平方英尺的实验室组成 加州大学洛杉矶分校的消化疾病分部与沃尔什博士的办公室附近。这 实验室的当前功能已完全配备。以前生产 董事批准后，根据要求很容易分发抗体。 准备新抗体的要求需要咨询 董事确定项目的可行性和最实用的 方法。大量中心调查员以及科学家 在全球范围内提供了核心服务，许多论文源于 与此核心的交互。在1993年至1998年6月之间，有87篇论文 来自中心是由与此核心的相互作用产生的。在 时期，调查人员还发表了44篇论文 治愈的成员，但使用了核心服务。