CORE--MORPHOLOGY/IMAGING

描述(改编自应用程序)此核心的总体目标是 通过继续为治疗中心计划提供 治愈那些拥有独立资助的研究项目的调查人员 形态、成像和功能方面的设施、培训和协助 白纹伊蚊的递质、受体、通道和其他整合蛋白的研究 天然细胞和转基因细胞。核心继续提供以下服务 可视化蜂窝组织和功能。其中包括一组 当代具有广泛应用的形态和成像方法 在细胞生物学和神经生物学方面，并将增加新的或改进的成像 促进信号转导研究的技术 在过去几年中发展起来的。以下服务将是 包括：A.用于确定变化的分离细胞悬浮液的荧光测定法 在细胞内有钙、氢或其他可用荧光探针分析的离子； 递质、激素、受体定位的免疫组织化学研究 和亚细胞和细胞水平的转运蛋白；C.轴突追踪 神经通路支配消化系统和控制消化系统 职能；d.协助和培训光学显微镜和光学显微镜； E.计算机辅助图像处理和分析 一种特定物质的表达变化的测定，以及对 不同细胞群的形态计量学分析；F.共聚焦显微镜 配备紫外光激光用于分析细胞内涉及的途径 细胞信号和蛋白质的细胞和亚细胞定位；这 系统还将允许直接重建 已识别的神经末梢和已定义的靶点.细胞内的G.离子成像 荧光探针用于分析单个细胞内的信号转导途径 细胞。整个系统已经升级；H.荧光成像系统用于 单激发双激发法体外测定细胞内cAMP水平 单电池中的发射配置。介绍一种微量注射系统 非故意的染料和非故意的信号抑制或促进剂 直接显示细胞内信号的细胞转导途径 Events可与荧光成像系统一起使用；I. 合理预测蛋白质折叠的分子模型，无论是在 膜蛋白的膜或膜外结构域，它 允许预测可访问的抗原点或模拟 突变。

DESCRIPTION (Adapted from the application) The overall goal of this core is to enhance the effectiveness of the CURE center program by continuing to provide CURE investigators who have independently-funded research projects with facilities, training and assistance for morphological, imaging and functional studies of transmitters, receptors, channels and other integral proteins in native and transfected cells. The core continues to offer services for visualizing cellular organizations and functions. These include an array of contemporary morphologic and imaging approaches that have a broad application in cell biology and neurobiology and will add new or improved imaging technologies to facilitate investigations in signal transduction that have developed during the past several years. The following services will be included: A. Fluorometry of isolated cell suspensions for determining changes in cell Ca2+, H+ or other ions accessible to fluorescent probe analysis; B. Immunohistochemistry for the localization of transmitters, hormones, receptors and transporters at the subcellular and cellular levels; C. Axonal tracing of neuronal pathways innervating the digestive system and controlling digestive functions; D. Assistance and training for light microscopy and photomicroscopy; E. Computer assisted image processing and analysis for quantitative determinations of changes in the expression of a given substance and for morphometric analysis of different cell populations; F. Confocal microscopy equipped with UV laser for the analysis of intracellular pathways involved in cellular signaling and cellular and subcellular localization of proteins; this system will also allow for a direct reconstruction of the relationship between identified nerve terminals and defined targets; G. Ion imaging of intracellular fluorescent probes for the analysis of signal transduction pathways in single cells. This entire system has been upgraded; H. Fluorescent imaging systems for in vitro measurement of intracellular cAMP levels using single excitation dual emission configuration in single cells. A microinjection system to introduce non-permeant dyes and non-permeant inhibitors or facilitators of signal transduction pathways into cells for the direct visualization of intracellular events is available in conjunction with the fluorescent imaging systems; I. Molecular modeling for a reasonable prediction of protein folding, either in the membrane or extramembrane domain of an integral membrane protein, which allows the prediction of accessible antigenic sites or to model the effects of mutations.