CORE F: MORPHOLOGY AND IMAGING

核心 F：形态学和成像

• 批准号: 7415063
• 负责人: CATIA STERNINI
• 金额: $ 7.52万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7415063
• 关键词: 3-Dimensional; Affinity; Animal Model; Animals; Archives; Area; Arts; Biochemical; Brain; Buffers; Cell membrane; Cells; Cellular biology; Cerebrum; Chemicals; Classification; Cognitive; Complement; Computer software; Computer-Assisted Image Processing; Condition; Confocal Microscopy; Consult; Consultations; Core Facility; Count; Cyclic AMP; Data Analyses; Digital Photography; Dimensions; Dyes; Educational process of instructing; Effectiveness; Electron Microscopy; Endocrine; Equipment; Evaluation; Event; Fluorescent Probes; Functional Imaging; Funding; Gene Expression; Genetically Engineered Mouse; Goals; Growth Factor; Health Services Accessibility; Healthcare Systems; Hormone Receptor; Human; Human Resources; Image; Image Analysis; Imagery; Imaging technology; Immunohistochemistry; In Vitro; Individual; Institution; Invasive; Investigation; Label; Laboratories; Lasers; Learning; Length; Licensing; Life; Ligands; Los Angeles; Measurement; Measures; Mediation; Metabolic; Metabolism; Methodology; Methods; Microinjections; Microscope; Modality; Modeling; Morphology; Mus; Neurobiology; Neurons; Opioid; Optics; Pathologist; Pathology; Pathway interactions; Patients; Performance; Perfusion; Personnel Staffing; Physiological; Plastics; Population; Positron-Emission Tomography; Preparation; Procedures; Proteins; Quartz; Range; Reagent; Recycling; Research Design; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Resources; SAS; Secure; Sensory Receptors; Services; Signal Transduction; Signal Transduction Pathway; Sorting - Cell Movement; Staining method; Stains; Statistical Models; Stimulus; Stress; Surface; Syndrome; System; Technical Expertise; Techniques; Technology; Temperature; Time; Tissue Procurements; Tissues; Training; Update; Villus; Visceral; Vision; cell preparation; cellular imaging; chronic pain; cost; density; detector; experience; fluorophore; genetic manipulation; glucose metabolism; improved; inhibitor/antagonist; interest; intracellular protein transport; investigator training; light microscopy; morphometry; mouse model; neuroimaging; neurotransmitter release; novel; programs; protein localization location; receptor; reconstruction; response; sample fixation; single photon emission computed tomography; skills; spatial relationship; tissue fixing; tissue processing; trafficking; user friendly software

The overall goal of the Morphology and Imaging Core is to enhance the effectiveness of the CURE: DDRCC program by continuing to provide CURE: DDRCC investigators who have independently-funded research projects with facilities, training and assistance for morphological, imaging and functional studies of transmitters, receptors, channels and other integral proteins in native and transfected cells. The Core continues to offer services for visualizing cellular and tissue organization and function. These include an array of contemporary morphologic and imaging approaches that have a broad application in cell biology and neurobiology and will add new or improved imaging technologies to facilitate investigations in signal transduction that have developed during the past several years. In addition, animal pathology service will be available to determine possible phenotypical alterations and pathological conditions that may be associated with genetically manipulated mice that are increasingly used by most CURE: DDRCC investigators. Furthermore, access to facilities within the institution for non-invasive neuroimaging will also be available through the Core for the increasing demand of technologies to investigate brain changes in stress-related pathologies. The Core will supply expertise and technical support, and will coordinate the imaging resources available at UCLA and the VA Greater Los Angeles Healthcare System. The core will negotiate access to these resources and coordinate their utilization, secure slots for imaging sessions, provide technologist support as needed, offer access to centralized computational resources for image archiving, data analysis and physiological and statistical modeling, and provide consulting services for study design and data analysis. The following services will be included: Immunohistochemistry, Assistance and training for Light Microscopy and Digital Photography, Computer-assisted image processing and analysis, Confocal microscopy, Ca2+ imaging, Fluorescent imaging system for in vitro measurement of intracellular cAMP levels, Animal pathology service, and Access to facilities for non-invasive neuroimaging approaches.

形态学和成像核心的总体目标是通过继续为CURE：DDRCC研究人员提供设施，培训和援助，以提高CURE：DDRCC计划的有效性，这些研究人员拥有独立资助的研究项目，用于天然和转染细胞中递质，受体，通道和其他整合蛋白的形态学，成像和功能研究。The Core继续提供可视化细胞和组织和功能的服务。这些包括一系列当代形态学和成像方法，这些方法在细胞生物学和神经生物学中具有广泛的应用，并将增加新的或改进的成像技术，以促进对细胞生物学和神经生物学的研究。 在过去的几年中已经发展起来的信号转导。此外，动物病理学服务将可用于确定可能与大多数CURE：DDRCC研究者越来越多地使用的遗传操作小鼠相关的可能表型改变和病理状况。此外，还将通过核心项目提供机构内的非侵入性神经成像设施，以满足日益增长的调查与压力有关的病理学大脑变化的技术需求。该中心将提供专业知识和技术支持，并将协调加州大学洛杉矶分校和VA大洛杉矶医疗保健系统的成像资源。芯会 协商对这些资源的访问并协调其利用，确保成像会话的插槽，根据需要提供技术人员支持，提供对集中计算资源的访问，以进行图像存档，数据分析以及生理和统计建模，并为研究设计和数据分析提供咨询服务。将提供以下服务：免疫组织化学、光学显微镜和数码摄影的协助和培训、计算机辅助图像处理和分析、共聚焦显微镜、Ca2+成像、用于体外测量细胞内cAMP水平的荧光成像系统、动物病理学服务以及使用非侵入性神经成像方法的设施。