CORE--MORPHOLOGY/IMAGING

核心——形态学/成像

• 批准号: 6410316
• 负责人: CATIA STERNINI
• 金额: $ 14.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410316
• 关键词: bioimaging /biomedical imaging; biomedical facility; computer simulation; fluorescent dye /probe; fluorimetry; gastrointestinal function; image processing; immunocytochemistry; microscopy; morphometry

DESCRIPTION (Adapted from the application) The overall goal of this core is to enhance the effectiveness of the CURE center program by continuing to provide CURE investigators who have independently-funded research projects with facilities, training and assistance for morphological, imaging and functional studies of transmitters, receptors, channels and other integral proteins in native and transfected cells. The core continues to offer services for visualizing cellular organizations and functions. These include an array of contemporary morphologic and imaging approaches that have a broad application in cell biology and neurobiology and will add new or improved imaging technologies to facilitate investigations in signal transduction that have developed during the past several years. The following services will be included: A. Fluorometry of isolated cell suspensions for determining changes in cell Ca2+, H+ or other ions accessible to fluorescent probe analysis; B. Immunohistochemistry for the localization of transmitters, hormones, receptors and transporters at the subcellular and cellular levels; C. Axonal tracing of neuronal pathways innervating the digestive system and controlling digestive functions; D. Assistance and training for light microscopy and photomicroscopy; E. Computer assisted image processing and analysis for quantitative determinations of changes in the expression of a given substance and for morphometric analysis of different cell populations; F. Confocal microscopy equipped with UV laser for the analysis of intracellular pathways involved in cellular signaling and cellular and subcellular localization of proteins; this system will also allow for a direct reconstruction of the relationship between identified nerve terminals and defined targets; G. Ion imaging of intracellular fluorescent probes for the analysis of signal transduction pathways in single cells. This entire system has been upgraded; H. Fluorescent imaging systems for in vitro measurement of intracellular cAMP levels using single excitation dual emission configuration in single cells. A microinjection system to introduce non-permeant dyes and non-permeant inhibitors or facilitators of signal transduction pathways into cells for the direct visualization of intracellular events is available in conjunction with the fluorescent imaging systems; I. Molecular modeling for a reasonable prediction of protein folding, either in the membrane or extramembrane domain of an integral membrane protein, which allows the prediction of accessible antigenic sites or to model the effects of mutations.

描述（改编自应用程序）该核心的总体目标是 通过继续提供以下服务来提高CURE中心计划的有效性 CURE研究人员拥有独立资助的研究项目， 形态、成像和功能方面的设施、培训和援助 研究递质，受体，通道和其他整合蛋白质， 天然和转染的细胞。核心继续提供服务， 可视化细胞组织和功能。其中包括一个数组 当代形态学和成像方法具有广泛的应用， 在细胞生物学和神经生物学中， 促进信号转导研究的技术， 在过去的几年里发展起来的。以下服务将 包括：A.分离的细胞悬液的荧光测定法用于测定变化 细胞中Ca 2+、H+或其它可用于荧光探针分析离子; B. 用于递质、激素、受体定位的免疫组织化学 以及亚细胞和细胞水平的转运蛋白; C.轴突追踪 支配消化系统和控制消化的神经元通路 功能; D.光学显微镜和显微照相术方面的援助和培训; E.计算机辅助图像处理和分析， 测定给定物质表达的变化， 不同细胞群体的形态计量学分析; F.共聚焦显微镜 配备了紫外激光器，用于分析细胞内途径， 细胞信号传导和蛋白质的细胞和亚细胞定位;这 系统还将允许直接重建 识别神经末梢和确定的靶点; G.细胞内离子成像 荧光探针用于分析信号转导途径， 细胞整个系统已经升级; H.荧光成像系统 使用单激发双重体外测量细胞内cAMP水平 单细胞中的发射构型。一种显微注射系统， 非渗透性染料和非渗透性信号抑制剂或促进剂 转导途径进入细胞，用于直接观察细胞内 事件可与荧光成像系统结合使用; I. 合理预测蛋白质折叠的分子建模，无论是在 整合膜蛋白的膜或膜外结构域， 允许预测可接近的抗原位点或模拟 突变。