LOSS OF NEGATIVE SIGNALING BY THE G-CSFR IN AML

AML 中 G-CSFR 负信号丢失

• 批准号: 6174121
• 负责人: Belinda Rene Avalos
• 金额: $ 20.21万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-23 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6174121
• 关键词: acute myelogenous leukemia; biological signal transduction; carcinogenesis; cell proliferation; clinical research; colony stimulating factor; cytokine receptors; gene expression; granulocyte; human subject; laboratory mouse; leukemia; point mutation; transfection

DESCRIPTION: (adapted verbatim from the investigator's abstract) Although considerable advances have been made in understanding growth inhibitory signaling by receptors that regulate immune responses, little is known about the mechanisms that mediate "negative" signaling by hematopoietic cytokine receptors. Recently, point mutations in the granulocyte colony-stimulating factor receptor (G-CSFR) gene deleting the distal growth inhibitory domain of the G-CSFR have been detected in patients with acute myelogenous leukemia (AML) that result in hypersensitivity to G-CSF and enhanced cell proliferation. These mutations have been identified in 100% of patients with AML preceded by server congenital neutropenia (SCN). The universal occurrence of these mutations in the subset of patients with SCN transforming to AML provides strong evidence that they contribute to leukemic transformation in this subset of patients. The precise nature of the growth inhibitory signals derived from the G-CSFR which are disrupted in patients with AML remain unknown. Additionally, the role of G-CSFR mutations in the development of AML in patients without antecedent SCN has not been adequately studied. Experiments are proposed here to better understand the mechanisms that regulate negative signaling by the G-CSFR and the contribution of G-CSFR mutations to leukemogenesis. This information will provide further insights into etiologic mechanisms underlying AML, permit the formulation of better defined guidelines for appropriate clinical use of G-CSF in patients with AML, and may reveal novel therapeutics targets for the treatment of AML.

描述：(逐字改编自调查人员的摘要) 在理解生长抑制方面已经取得了相当大的进展 调节免疫反应的受体发出的信号，人们对此知之甚少 造血细胞因子介导“负性”信号的机制 感受器。最近，粒细胞集落刺激因子中的点突变 因子受体(G-CSFR)基因缺失的远端生长抑制域 在急性髓系白血病(AML)患者中检测到G-CSFR 这会导致对G-CSF的超敏反应，并促进细胞增殖。这些 SERVER先于AML的患者中已发现100%的突变 先天性中性粒细胞减少症(SCN)这些突变普遍存在于 SCN转化为AML的患者亚群提供了强有力的证据 它们有助于这一亚群患者的白血病转化。 来自G-CSFR的生长抑制信号的精确性质 在急性髓系白血病患者中，这些基因的表达被打乱仍不清楚。此外，这个角色 G-CSFR突变在无先证者急性髓系白血病发病中的作用 SCN还没有得到充分的研究。这里提出的实验是为了更好地 了解G-CSFR和G-CSFR调节负面信号的机制 G-CSFR突变在白血病发生中的作用。此信息将 为急性髓细胞白血病的病因机制提供进一步的见解，允许 制定更明确的指南，以便临床适当使用G-CSF 在急性髓系白血病患者中，并可能揭示治疗急性髓细胞白血病的新的治疗靶点 急性髓系白血病的治疗。