MECHANICALLY GUIDED TISSUE REGENERATION

描述 (改编自申请人的摘要)这项研究的目的是 检验组织的整合和功能的全局假设 骨再生的工程化结构受到以下因素的显著影响 通过细胞外基质传递物理力量。 利用一系列体内老鼠模型，研究人员将测试 装置介导的机械力和局部力的协同效应 刺激修复和整合的生物因素的传递 组织工程化骨替代物。无论是头面部还是面部的模特 长骨部位使用专门设计的外固定器，使 实验控制机械加载的实现 手术造成的截骨间隙的情况。局部生物因素 将DNA输送基质放置在这些骨折内而产生 差距。机械和生物因素对组织修复的影响 并将通过记录基因的空间模式来测试再生 表达、基质合成和骨骼形态随时间和时间的变化 解剖位置。 这些信息是制定替代战略的基础 或增加头面部或附件骨骼的骨缺陷。 确定影响合并和重塑的因素 组织工程化移植将带来显著的改善和 更可靠的仿生疗法。

DESCRIPTION (Adapted from the applicant's abstract) The purpose of this research is to test the global hypothesis that the incorporation and function of a tissue engineered construct for bone regeneration is significantly influenced by the transmission of physical forces through its extracellular matrix. Utilizing a series of in vivo rat models, the investigators will test for the synergistic affects of device mediated mechanical forces and local delivery of biologic factors in stimulating the repair and incorporation of tissue engineered bone substitutes. The models in both a cranial-facial and long bone site utilize specifically designed external fixators which enable the implementation of experimentally controlled mechanical loading conditions across surgically created osteotomy gaps. Local biologic factors will be produced by placing DNA-delivering matrices within these fracture gaps. The effects of the mechanical and biologic factors on tissue repair and regeneration will be assayed by documenting the spatial patterns of gene expression, matrix synthesis, and bone morphology as a function of time and anatomic location. This information is fundamental to the development of strategies to replace or augment bony defects in the cranial-facial or appendicular skeleton. Identification of factors which influences the incorporation and remodeling of tissue engineered transplants will lead to significantly improved and more reliable biomimetic therapies.