THE ROLE OF SEQA PROTEIN IN E.COLI REPLICATION CONTROL

SECA 蛋白在大肠杆菌复制控制中的作用

• 批准号: 6208152
• 负责人: David Bates
• 金额: $ 3.24万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6208152
• 关键词: DNA footprinting; DNA methylation; DNA replication; DNA replication origin; Escherichia coli; bacterial proteins; biomedical resource; biotechnology; cell cycle; crosslink; fluorescence microscopy; fluorescent in situ hybridization; green fluorescent proteins; immunoprecipitation; mass tissue /cell culture; protein binding; protein structure function; technology /technique development; transcription factor

It is the long-term goal of this research proposal to determine how the rate of DNA replication is coordinated to the cell division cycle in the model organism of E. coli. Understanding these events in bacteria is essential to guide future cell-cycle research in humans. To achieve this goal, this research project is designed to resolve two questions about replication control in e. COLI: First, what is the role of SeqA protein in regulating initiation of DNA replication, and specifically, what is its role in controlling origin movement within the cell? Second, what is the relationship between the sequence of events occurring at replication termiantion in part controlled by SeqA, and the timing of replication initiation? These questions will be addressed by examining the timing of varius events, by fluorescence microscopy and molecular methods, that occur at and immediately and mutant cells that are defective in the control of replication initiation. In the process of this investigation, we will develop a new method to obtain synchronized cultures.

本研究计划的长期目标是确定大肠杆菌模式生物中 DNA 复制速率如何与细胞分裂周期相协调。了解细菌中的这些事件对于指导未来的人类细胞周期研究至关重要。为了实现这一目标，该研究项目旨在解决有关 e 中复制控制的两个问题。 COLI：首先，SeqA 蛋白在调节 DNA 复制起始中的作用是什么？具体来说，它在控制细胞内起源运动方面的作用是什么？其次，部分由 SeqA 控制的复制终止时发生的事件序列与复制启动的时间之间有什么关系？这些问题将通过荧光显微镜和分子方法检查各种事件的时间来解决，这些事件立即发生在复制起始控制方面存在缺陷的突变细胞。在本次研究的过程中，我们将开发一种获得同步培养物的新方法。