Next-generation epigenetic analysis: direct reading of DNA methylation

下一代表观遗传分析：直接读取 DNA 甲基化

• 批准号: DP220102086
• 负责人: Prof Yuling Wang
• 金额: $ 28.05万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-05-18 至 2026-05-17
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP220102086
• 关键词: Next; generation; epigenetic; analysis; direct

This project aims to develop a new molecular tool to directly and dynamically read chemical modifications on genomic DNA (epigenetics) by utilizing advanced nanomaterials with the unique features of Raman spectroscopy. Epigenetics affects cellular processes and controls genetic programs by turning them “on” and “off" but there is currently no direct method to measure modifications on DNA. A new technology will be designed to avoid complicated procedures/chemistry for DNA epigenetic analysis providing a specific molecular fingerprint. The anticipated outcomes include a new technique and advanced knowledge in nanomaterials and DNA functions, thus strengthening the economic viability of Australian manufacturing and biotechnology sectors.

该项目旨在开发一种新的分子工具，通过利用具有拉曼光谱独特功能的先进纳米材料，直接和动态地读取基因组DNA（表观遗传学）上的化学修饰。表观遗传学影响细胞过程，并通过“打开”和“关闭”来控制遗传程序，但目前还没有直接的方法来测量DNA上的修饰。将设计一种新技术，以避免DNA表观遗传分析的复杂程序/化学，提供特定的分子指纹。预期成果包括纳米材料和DNA功能方面的新技术和先进知识，从而加强澳大利亚制造业和生物技术部门的经济可行性。