THE ROLE OF SEQA PROTEIN IN E.COLI REPLICATION CONTROL

SECA 蛋白在大肠杆菌复制控制中的作用

• 批准号: 6385226
• 负责人: David Bates
• 金额: $ 4.02万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6385226
• 关键词: DNA footprinting; DNA methylation; DNA replication; DNA replication origin; Escherichia coli; bacterial proteins; biomedical resource; biotechnology; cell cycle; crosslink; fluorescence microscopy; fluorescent in situ hybridization; green fluorescent proteins; immunoprecipitation; mass tissue /cell culture; protein binding; protein structure function; technology /technique development; transcription factor

It is the long-term goal of this research proposal to determine how the rate of DNA replication is coordinated to the cell division cycle in the model organism of E. coli. Understanding these events in bacteria is essential to guide future cell-cycle research in humans. To achieve this goal, this research project is designed to resolve two questions about replication control in e. COLI: First, what is the role of SeqA protein in regulating initiation of DNA replication, and specifically, what is its role in controlling origin movement within the cell? Second, what is the relationship between the sequence of events occurring at replication termiantion in part controlled by SeqA, and the timing of replication initiation? These questions will be addressed by examining the timing of varius events, by fluorescence microscopy and molecular methods, that occur at and immediately and mutant cells that are defective in the control of replication initiation. In the process of this investigation, we will develop a new method to obtain synchronized cultures.

这项研究提案的长期目标是确定DNA复制速率如何与大肠杆菌模型生物体中的细胞分裂周期协调。了解细菌中的这些事件对于指导人类的未来细胞周期研究至关重要。为了实现这一目标，该研究项目旨在解决e中有关复制控制的两个问题。大肠杆菌：首先，Seqa蛋白在调节DNA复制的开始中的作用是什么，具体来说，它在控制细胞内的起源运动中的作用是什么？其次，在复制术语处发生的事件序列部分由SEQA控制，而复制启动的时间是什么？这些问题将通过通过荧光显微镜和分子方法来检查瓦里乌斯事件的时机，并立即发生，而突变细胞在控制复制起始中有缺陷。在这项研究的过程中，我们将开发一种新方法来获得同步文化。

项目成果

The bacterial replisome: back on track?

• DOI: 10.1111/j.1365-2958.2008.06378.x
• 发表时间: 2008-09-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Bates, David

The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication.

• DOI: 10.1111/j.1365-2958.2009.06877.x
• 发表时间: 2009-10
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Stepankiw N;Kaidow A;Boye E;Bates D
• 通讯作者: Bates D