VASCULAR PATTERNING IN LEAVES OF ARABIDOPSIS THALIANA

我将研究PROV1在拟南芥叶片维管模式形成中的作用。叶片的维管模式是由位置线索决定的，而发育中的叶脉本身可能作为叶片中细胞类型的后续模式的线索。基因PROV1最近被发现，使用增强子陷阱策略，它在年轻的血管前细胞中特异性表达，当克隆时被发现编码受体样蛋白激酶。PROV1在成熟植物维管形成和分化中的功能将通过PROV1过表达、过表达和有条件表达来研究。激酶活性将被评估，亚细胞定位将通过原位免疫定位确定，相互作用蛋白将通过酵母双杂交系统中的异源表达来鉴定。研究PROV1在静脉发育中的作用以及对静脉模式形成的调控机制的深入了解，将为理解植物器官发生过程中的位置规范带来有价值的进展。

I will investigate the role of PROV1 in vascular pattern formation in leaves of Arabidopsis thaliana. Vascular patterning in leaves is specified by positional cues and the developing veins themselves likely act as cues for the subsequent patterning of cell types in the leaf. The gene PROV1 was recently identified, using an enhancer trap strategy, for its specific expression in young provascular cells and when cloned was found to encode a receptor-like-protein-kinase. The function of PROV1 in vascular specification and differentiation will be addressed by over-, under-, and conditionally expressing PROV1 in mature plants. The kinase activity will be evaluated, the subcellular localization will be determined by imunolocalization in situ, and interacting proteins will be identified using heterologous expression in the yeast two-hybrid system. The characterization of the role of PROV1 in vein development and insight into mechanisms which regulate vein pattern formation will result in valuable progress toward understanding positional specification during plant organogenesis.