GAP JUNCTIONS ROLE IN MATURATION OF GRANULATION TISSUE

拟议的项目有三个具体目标。首先，证明缝隙连接在器官培养的胎鼠伤口成纤维细胞组织和肉芽组织的组织学成熟中起作用。其次，建立一个体内模型，证明在分阶段小鼠皮瓣的肉芽组织中存在间隙连接。第三，在体内小鼠模型中，间隙连接有助于肉芽组织的成熟。第一个目的将通过Western blot分析和培养的胎鼠前肢切口的连续组织学切片来实现。第二个目标将通过向分阶段小鼠皮瓣的成纤维细胞中微量注射染料来实现。最终目的将通过间隙连接解耦剂对体内肉芽组织组织学成熟的影响来实现。拟议的项目将帮助研究人员和临床医生更好地了解伤口愈合的细胞方面。有了这些信息，就可以实现更好的伤口护理和患者护理。

The proposed project has three specific aims. First, to show that gap junctions play a role in fibroblast organization and histological maturation of granulation tissue in organ cultured fetal mouse wounds. Second, to develop an in vivo model that demonstrates the presence of gap junctions in granulation tissue of staged mouse skin flaps. Third, to show that gap junctions in an in vivo mouse model are instrumental in maturation of granulation tissue. The first aim will be achieved through Western blot analysis and serial histological sections of an incisional wound made in cultured fetal mouse forelimbs. The second aim will be achieved through microinjection of dye into fibroblasts of a staged mouse skin flap. The final aim will be achieved through the effects of gap junction uncouplers on the histological maturation of granulation tissue in vivo. The proposed project will help both researchers and clinicians to better understand the cellular aspects of wound healing. With this information, better wound care and therefore patient care can be achieved.