GAP JUNCTIONS ROLE IN MATURATION OF GRANULATION TISSUE

拟议的项目有三个具体目标。首先，证明缝隙连接在器官培养的胎鼠创面成纤维细胞组织和肉芽组织的组织成熟中起作用。第二，开发一种体内模型，证明在分期的小鼠皮瓣的肉芽组织中存在缝隙连接。第三，为了证明在活体小鼠模型中的缝隙连接有助于肉芽组织的成熟。第一个目标将通过Western印迹分析和对培养的胎鼠前肢切开伤口的连续组织切片来实现。第二个目标将通过将染料显微注射到分期小鼠皮瓣的成纤维细胞中来实现。最终的目的将通过缝隙连接解偶联剂对体内肉芽组织的组织成熟的影响来实现。拟议的项目将帮助研究人员和临床医生更好地了解伤口愈合的细胞学方面。有了这些信息，可以实现更好的伤口护理，从而实现对病人的护理。

The proposed project has three specific aims. First, to show that gap junctions play a role in fibroblast organization and histological maturation of granulation tissue in organ cultured fetal mouse wounds. Second, to develop an in vivo model that demonstrates the presence of gap junctions in granulation tissue of staged mouse skin flaps. Third, to show that gap junctions in an in vivo mouse model are instrumental in maturation of granulation tissue. The first aim will be achieved through Western blot analysis and serial histological sections of an incisional wound made in cultured fetal mouse forelimbs. The second aim will be achieved through microinjection of dye into fibroblasts of a staged mouse skin flap. The final aim will be achieved through the effects of gap junction uncouplers on the histological maturation of granulation tissue in vivo. The proposed project will help both researchers and clinicians to better understand the cellular aspects of wound healing. With this information, better wound care and therefore patient care can be achieved.