GAP JUNCTIONS ROLE IN MATURATION OF GRANULATION TISSUE

间隙连接在肉芽组织成熟中的作用

• 批准号: 6385211
• 负责人: KURTIS E MOYER
• 金额: $ 4.2万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-27 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6385211
• 关键词: biological models; cell cycle; dyes; fibroblasts; gap junctions; granulocyte; histogenesis; laboratory mouse; laboratory rat; limbs; microinjections; model design /development; newborn animals; organ culture; skin; western blottings; wound healing

The proposed project has three specific aims. First, to show that gap junctions play a role in fibroblast organization and histological maturation of granulation tissue in organ cultured fetal mouse wounds. Second, to develop an in vivo model that demonstrates the presence of gap junctions in granulation tissue of staged mouse skin flaps. Third, to show that gap junctions in an in vivo mouse model are instrumental in maturation of granulation tissue. The first aim will be achieved through Western blot analysis and serial histological sections of an incisional wound made in cultured fetal mouse forelimbs. The second aim will be achieved through microinjection of dye into fibroblasts of a staged mouse skin flap. The final aim will be achieved through the effects of gap junction uncouplers on the histological maturation of granulation tissue in vivo. The proposed project will help both researchers and clinicians to better understand the cellular aspects of wound healing. With this information, better wound care and therefore patient care can be achieved.

拟议项目有三个具体目标。 首先，证明间隙连接在器官培养胎鼠伤口的成纤维细胞组织和肉芽组织的组织学成熟中发挥作用。 其次，开发一个体内模型，证明分阶段小鼠皮瓣的肉芽组织中存在间隙连接。 第三，证明体内小鼠模型中的间隙连接有助于肉芽组织的成熟。第一个目标将通过对培养的胎儿小鼠前肢切口进行蛋白质印迹分析和连续组织学切片来实现。 第二个目标将通过将染料显微注射到阶段性小鼠皮瓣的成纤维细胞中来实现。 最终目标将通过间隙连接解偶联剂对体内肉芽组织的组织学成熟的影响来实现。 拟议的项目将帮助研究人员和临床医生更好地了解伤口愈合的细胞方面。 有了这些信息，就可以实现更好的伤口护理，从而实现患者护理。