CLONING AND CHARACTERIZATION OF AN IL-1-LIKE GENE
CLONING AND CHARACTERIZATION OF AN IL-1-LIKE GENE
IL-1 样基因的克隆和表征
批准号：
6062491
负责人：
KRISTINA M WASSON-BLADER
金额：
$2.51万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
项目状态：
未结题
起止时间：
2000-02-01 至
关键词：
antibody formation artificial chromosomes complementary DNA cytokine receptors evolution follicle stimulating hormone fusion gene gene expression hormone regulation /control mechanism immunocytochemistry in situ hybridization interleukin 1 laboratory rat natural gene amplification northern blottings ovary progesterone protein structure function receptor binding recombinant proteins sequence tagged sites tissue /cell culture
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DESCRIPTION The proposed research will clone and characterize a novel IL-1 like gene (IL-1T), which was amplified from human ovary cDNA library. Following characterization of this gene, tissue-and cell-specific expression of the IL-1T will be analyzed using Northern blot analysis and in situ hybridization studies. A fusion protein will be generated for antibody production and for use in protein expression studies using immunohistochemical techniques. In addition, the recombinant protein will be produced and used in assays to determine receptor binding affinities and biological activities. The proposed study will identify the function(s) of this IL-1 like gene and assist in the understanding of the potential role(s) of cytokines in ovarian physiology.
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