PHYSIOLOGY OF INHIBITORY SYNAPSES IN THE VISUAL CORTEX

该提案的长期目标是分析 大鼠脑内GABA介导的突触连接的特性和调控 并将这些特性与抑制作用联系起来 在大脑皮层信息处理中。我们将使用双重录音，从 两个突触连接的神经元以及记录的 微型IPSCs及其激动剂快速应用技术 切除斑块来研究两个主要的特定目标：1.单一抑制 GABA能突触配对记录将被用来估计 视皮层抑制性突触传递的可靠性。 第二个目标是评估单个抑制性神经元的影响 它们突触后靶点的活动。第三个目标是 研究不同脑区神经元突触反应的差异 抑制性神经元的类别。我们将研究突触后反应 由轴-体和轴-树突起激活产生 GABA能神经元。2.微型IPSCs的GABAA受体记录 联合GABA快速应用于切除的外向外补片 将用于研究GABAA受体的功能特性 不同类型的锥体和非锥体神经元。第二个目标 是为了找出GABAA受体之间是否在功能上存在差异 不同的神经元隔间。我们将研究以下方面的反应 树突状、体细胞和轴突向外贴片快速应用 对GABA的影响。

The long-term goal of this proposal is to analyze the functional properties and modulation of GABA-mediated synaptic connections in the rat visual cortex and relate these properties to the role of inhibition in cortical processing of information. We will use dual recording from pairs of synaptically connected neurons together with recordings of miniature IPSCs and techniques for rapid application of agonist onto excised patches to study two main specific aims: 1. Unitary Inhibitory GABAergic Synapses Paired recording will be used to estimate the reliability of inhibitory synaptic transmission in the visual cortex. The second goal is to assess the impact of individual inhibitory neurons on the activity of their postsynaptic targets. The third goal is to study the differences of the synaptic responses generated by different classes of inhibitory neurons. We would study the postsynaptic response generated by activation of axo-axonic axo-somatic and axo-dendritic GABAergic neurons. 2. GABAA receptors Recording of miniature IPSCs together with rapid application of GABA to excised outside-out patches will be used to study the functional properties of GABAA receptors in different types of pyramidal and nonpyramidal neurons. The second goal is to find if there are functional differences among GABAA receptors at different neuronal compartments. We will study the response of dendritic, somatic and axonal outside-out patches to rapid application of GABA.