BIOLOGY AND FUNCTION OF LYMPHOCYTE ADHESION MOLECULES

淋巴细胞粘附分子的生物学和功能

• 批准号: 6125389
• 负责人: Ivan Stamenkovic
• 金额: $ 26.35万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6125389
• 关键词: B lymphocyte; CD antigens; CD5 molecule; T lymphocyte; biological signal transduction; cell adhesion; genetically modified animals; glycosylation; laboratory mouse; leukocyte adhesion molecules; ligands; molecular cloning; selectins; sialate; transfection

DESCRIPTION (Adapted from the Investigator's Abstract): Normal lymphocyte function is highly dependent on a variety of cell surface receptors that mediate cell-cell adhesion, and transmit contact-dependent activation signals to the cellular interior. The project described focuses on three adhesion-dependent responses in T and B cells. CD22 is a sialic acid-binding receptor that recognizes sIgM and CD45. This project will examine the role of sialylation of CD22 itself in the ligand-binding activity of this receptor. Wild-type and mutant forms of CD22 will be introduced into CD22 nullizygous B cells to determine whether alterations in the sialylation of the extracellular domain augments or interferes with the function of CD22. The investigator has identified a novel, inducible ligand for CD5 on activated B cells, and has shown that CD5 glycosylation regulates CD5 binding to this ligand. The investigator now plans to study the glycosylation of CD5 in detail, and to clone the novel CD5 ligand. Two cDNAs encoding putative ligands for the L-Selectin cytoplasmic domain have been cloned, and the objective of the third aim of this project is to characterize the cDNAs, and to examine their roles in L-Selectin-mediated adhesion and rolling.

描述（改编自研究者摘要）：正常淋巴细胞 功能高度依赖于多种细胞表面受体， 介导细胞间粘附，并传递接触依赖性激活 向细胞内部发送信号。 该项目描述的重点是三个 T和B细胞中的粘附依赖性应答。 CD 22是唾液酸 识别sIgM和CD 45的酸结合受体。 该项目将 检查CD 22本身的唾液酸化在配体结合中的作用， 该受体的活性。 CD 22的野生型和突变形式将被 导入CD 22缺失型B细胞中，以确定 细胞外结构域的唾液酸化增强或干扰细胞外结构域的功能， CD 22的功能 研究人员发现了一种新的可诱导配体 对于活化的B细胞上的CD 5，并且已经表明CD 5糖基化调节 CD 5与该配体的结合。 研究人员现在计划研究 本发明的目的在于详细地研究CD 5的糖基化，并克隆新的CD 5配体。 两 编码L-选择素胞质结构域的推定配体的cDNA具有 被克隆，该项目的第三个目标是 鉴定cDNA，并检查它们在L-选择素介导的 粘合和轧制。