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BIOLOGY AND FUNCTION OF LYMPHOCYTE ADHESION MOLECULES

淋巴细胞粘附分子的生物学和功能

基本信息

批准号：
6329739
负责人：
Ivan Stamenkovic
金额：
$ 26.98万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-08-01 至 2001-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6329739
关键词：
B lymphocyte CD antigens CD5 molecule T lymphocyte biological signal transduction cell adhesion genetically modified animals glycosylation laboratory mouse leukocyte adhesion molecules ligands molecular cloning selectins sialate transfection

项目摘要

DESCRIPTION (Adapted from the Investigator's Abstract): Normal lymphocyte function is highly dependent on a variety of cell surface receptors that mediate cell-cell adhesion, and transmit contact-dependent activation signals to the cellular interior. The project described focuses on three adhesion-dependent responses in T and B cells. CD22 is a sialic acid-binding receptor that recognizes sIgM and CD45. This project will examine the role of sialylation of CD22 itself in the ligand-binding activity of this receptor. Wild-type and mutant forms of CD22 will be introduced into CD22 nullizygous B cells to determine whether alterations in the sialylation of the extracellular domain augments or interferes with the function of CD22. The investigator has identified a novel, inducible ligand for CD5 on activated B cells, and has shown that CD5 glycosylation regulates CD5 binding to this ligand. The investigator now plans to study the glycosylation of CD5 in detail, and to clone the novel CD5 ligand. Two cDNAs encoding putative ligands for the L-Selectin cytoplasmic domain have been cloned, and the objective of the third aim of this project is to characterize the cDNAs, and to examine their roles in L-Selectin-mediated adhesion and rolling.

描述(改编自《调查者摘要》)：正常淋巴细胞功能高度依赖于多种细胞表面受体，这些受体介导细胞间的黏附，传递接触依赖的激活发往细胞内部的信号。所描述的项目集中在三个方面 T细胞和B细胞的黏附依赖反应。CD22是唾液酸词识别SIGM和CD45的酸结合受体。这个项目将检测CD22自身唾液酸化在配体结合中的作用这种受体的活性。野生型和突变型CD22将是将其导入CD22无合子B细胞，以确定在胞外区的唾液酸化增加或干扰 CD22的功能。研究人员发现了一种新的、可诱导的配体对于活化的B细胞上的CD5，并且已经表明CD5糖基化调节 CD5与该配体结合。调查员现在计划研究对CD5的糖基化进行了详细的研究，并克隆了新型CD5配体。二编码L-选择素胞质结构域的假定配体的cDNA有已经被克隆，这个项目的第三个目标是鉴定cDNA，并研究它们在L-选择素介导中的作用粘合和滚动。