REGULATORY MECHANISMS OF APOPTOTIC CELL DEATH

凋亡细胞死亡的调控机制

• 批准号: 6019146
• 负责人: Ivan Stamenkovic
• 金额: $ 24.12万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019146
• 关键词: antioxidants; apoptosis; cell cycle; cell line; cytogenetics; enzyme activity; free radical oxygen; oxidation reduction reaction; protooncogene; superoxide dismutase

The mechanisms that mediate cellular sensitivity to apoptotic stimuli are poorly understood. Considerable data suggests that reactive oxygen intermediates (R01s) may play a critical role in this regard. For example, the PI has demonstrated that increases in intracellular 02- appears to protect cells from Fas-mediated death. In this proposal, the PI plans to: 1) use various agents to alter intracellular 02- production in a variety of different cells types stimulated to die so that the generalizability of this observation can determined; 2) examine the effects of redox balance on caspase activity; 3) determine if Bcl-2 alters redox state in cells; 4) evaluate the effects of altered redox state on apoptosis sensitivity of tumor cells in vivo; and 5) determine if activation-associated changes in lymphocyte sensitivity to apoptotic stimuli is redox state dependent.

调节细胞对凋亡刺激敏感性的机制有 人们对此知之甚少。大量数据表明，活性氧 中间体(R01)可能在这方面发挥关键作用。为 例如，PI已经证明细胞内02-增加- 似乎可以保护细胞免受Fas介导的死亡。在这项提案中， PI计划：1)使用各种药物改变细胞内02-产生 在各种不同类型的细胞中被刺激死亡，因此 可以确定这个观察的概括性；2)检查 氧化还原平衡对半胱氨酸天冬氨酸氨基转移酶活性的影响；3)确定Bcl2是否改变 细胞内氧化还原状态；4)评估氧化还原状态改变对 体内肿瘤细胞的凋亡敏感性；以及5)决定是否 淋巴细胞对凋亡敏感性的活化相关变化 刺激依赖于氧化还原状态。

项目成果

Thyroid carcinoma cells are resistant to FAS-mediated apoptosis but sensitive to tumor necrosis factor-related apoptosis-inducing ligand.

• 发表时间: 2000-08
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: N. Mitsiades;V. Poulaki;S. Tseleni-Balafouta;D. Koutras;I. Stamenkovic