MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM

盘基网柄菌细胞分裂的分子分析

• 批准号: 6200795
• 负责人: ARTURO DE LOZANNE
• 金额: $ 24.07万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6200795
• 关键词: Dictyostelium; actins; biological signal transduction; cell cycle; chimeric proteins; cytoplasm; cytoskeletal proteins; gene expression; gene mutation; gene targeting; genetically modified animals; guanosinetriphosphatases; molecular biology; molecular cloning; mutant; myosins; osmosis; phenotype; pinocytosis; polymerase chain reaction; protein purification; protein sequence; protein structure function; tissue /cell culture; yeast two hybrid system

DESCRIPTION: This investigator has made significant contributions to the understanding of cytokinesis. The mechanisms responsible for completing cytokinesis remain poorly defined. During the current period of support the applicant performed a genetic screen to identify novel proteins involved in cytokinesis. Two genes were identified. The first is racE, a small GTPase that appears to be an important signalling molecule required for cytokinesis. The second gene, called lvsA, has homology to the murine beige and the human FAN proteins, and is also required for cytokinesis. The current application builds on these findings with aims to dissect the role these two genes play in cytokinesis. Three specific aims are proposed: (1) to determine the role racE plays in cytokinesis, (2) to determine what other molecules interact with racE, and (3) to determine the role lvsA plays in cytokinesis.

描述：这位研究者对研究做出了重大贡献。 对胞质分裂的理解 负责完成的机制 胞质分裂仍不明确。 在目前的支助期间， 申请人进行了遗传筛选，以鉴定涉及 胞质分裂 鉴定了两个基因。 第一个是racE，一款小型的GTcycle 这似乎是一种重要的信号分子， 胞质分裂 第二个基因，称为lvsA，与鼠米色具有同源性， 和人FAN蛋白，并且也是胞质分裂所需的。 的 目前的应用程序建立在这些发现的基础上，旨在剖析 这两个基因在胞质分裂中起作用。 提出了三个具体目标：（1） 为了确定racE在胞质分裂中的作用，（2）为了确定其他的 分子与racE相互作用，以及（3）确定lvsA在 胞质分裂