MOLECULAR ANALYSIS OF CYTOKINESIS IN DICTYOSTELIUM

盘基网柄菌细胞分裂的分子分析

• 批准号: 6138461
• 负责人: ARTURO DE LOZANNE
• 金额: $ 24.59万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6138461
• 关键词: Dictyostelium; actins; biological signal transduction; cell cycle; chimeric proteins; cytoplasm; cytoskeletal proteins; gene expression; gene mutation; gene targeting; genetically modified animals; guanosinetriphosphatases; molecular biology; molecular cloning; mutant; myosins; osmosis; phenotype; pinocytosis; polymerase chain reaction; protein purification; protein sequence; protein structure function; tissue /cell culture; yeast two hybrid system

DESCRIPTION: This investigator has made significant contributions to the understanding of cytokinesis. The mechanisms responsible for completing cytokinesis remain poorly defined. During the current period of support the applicant performed a genetic screen to identify novel proteins involved in cytokinesis. Two genes were identified. The first is racE, a small GTPase that appears to be an important signalling molecule required for cytokinesis. The second gene, called lvsA, has homology to the murine beige and the human FAN proteins, and is also required for cytokinesis. The current application builds on these findings with aims to dissect the role these two genes play in cytokinesis. Three specific aims are proposed: (1) to determine the role racE plays in cytokinesis, (2) to determine what other molecules interact with racE, and (3) to determine the role lvsA plays in cytokinesis.

描述：该研究人员为研究做出了重大贡献 对胞质分裂的了解。 负责完成的机制 胞质分裂的定义仍然不明确。 在当前的支持期间 申请人进行了基因筛选，以鉴定涉及的新蛋白质 胞质分裂。 鉴定出两个基因。 第一个是racE，一种小型GTPase 这似乎是一个重要的信号分子 胞质分裂。 第二个基因称为 lvsA，与小鼠米色具有同源性 和人类 FAN 蛋白，也是胞质分裂所必需的。 这 当前的应用程序建立在这些发现的基础上，旨在剖析该角色 这两个基因在胞质分裂中发挥作用。 提出了三个具体目标：(1) 确定 racE 在胞质分裂中的作用，(2) 确定其他什么 分子与 racE 相互作用，并且 (3) 确定 lvsA 在 胞质分裂。