NONSENSE MEDIATED MRNA DECAY PATHWAY

描述：信使RNA（mRNA）降解是一个过程， 在基因表达调控中的重要作用。 mRNA的衰变率 并且可以响应于环境信号进行调制。 许多 研究表明，mRNA的更新与翻译有关。 有一种途径已经被广泛研究， 联系到翻译。 一种被广泛应用的途径 研究并清楚地阐明了翻译和mRNA之间的联系 周转是无义介导的mRNA衰变途径。 这笔赠款的主要目的如下。 第一，严格界定 无义密码子后刺激衰变所需的序列。 第二，进行几次基因筛查， 在无义介导的衰变中起作用的组分。 第三，利用A 一系列的遗传和生化分析，以收集有关 这些反式作用因子的结构和功能。

DESCRIPTION: Messenger RNA (mRNA) degradation is a process that plays an important role in the regulation of gene expression. mRNA decay rates vary greatly and can be modulated in response to environmental signals. Many studies have demonstrated that mRNA turnover can be linked to translation. One pathway that has been extensively investigated and clearly exemplifies the linked to translation. One pathway that has been extensively investigated and clearly exemplifies the link between translation and mRNA turnover is the nonsense-mediated mRNA decay pathway. The major aims of this grant are as follows. First, to rigorously define the sequences required for stimulation of decay following a nonsense codon. Second, to perform several genetic screens to identify trans acting components that function in nonsense mediated decay. Third, to utilize a series of genetic and biochemical assays to gather information about the structure and function of those trans-acting factors.