RNA POLYMERASE AND BACTERIAL DIFFERENTIATION

RNA 聚合酶和细菌分化

基本信息

  • 批准号:
    6133888
  • 负责人:
  • 金额:
    $ 33.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1996
  • 资助国家:
    美国
  • 起止时间:
    1996-06-01 至 2004-05-31
  • 项目状态:
    已结题

项目摘要

As the bacterium Bacillus subtilis differentiates from the vegetative form into a dormant endospore, complex morphological changes occur that require the expression of many genes. During the process, new RNA polymerase sigma subunits appear, displacing one another and conferring on the RNA polymerase different specificities for the recognition of different classes of promoters. The activity of each sigma factor appears to be regulated in response to morphological cues that signal the completion of specific stages in the differentiation process, and in several instances intercellular signaling between the forespore and mother cell is required. Elucidation of the mechanisms that synchronize morphological transformations and gene expression in Bacillus subtilis may lead to the discovery of novel mechanisms that regulate gene expression in a wide variety of microorganisms. Therefore, we will study the mechanisms that synchronize the activation of two different sigma factors with two different morphological transformations. SapA protein acts in the early stage of spore coat assembly and may be proteolytically processed by the same machinery that processes pro- to activate thereby coordinating the activation of sigma K with the completion of the foundation for the spore coat. We will test this hypothesis, and determine how SapA is targeted to the interface region between the undercoat and cortex peptidoglycan. Forespore-specific transcription is initiated by sigma F-RNA polymerase, and results in the forespore-specific production of sigmaG. However, transcription of spoIIIG, the structural gene for , is delayed relative to other sigmaF-dependent genes, and requires an unidentified intercellular signal. We will characterize this mechanism of intercellular regulation by identifying cis-acting elements in the spoIIIG promoter, and trans-acting factors that regulate spoIIIG promoter activity. sigmaG accumulates after transcription of spoIIIG but does not become fully active until engulfment of the forespore is completed. We will study the function of two proteins that appear to affect sigmaG activation. Transcription of the orfD gene, which encodes one of these proteins, is directed from a promoter unlike any other known to be active during sporulation. Therefore, we will elucidate the mechanism that regulates orfD transcription. Mutations in spoIIIJ prevent sigmaG activation. We will test the hypothesis that spoIIIJ encodes a pheromone-like peptide that plays a role in signaling sigmaG activation.
当枯草芽孢杆菌从营养形态分化成

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Charles P. Moran其他文献

Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene
枯草芽孢杆菌的两种 RNA 聚合酶西格玛因子区分发育调控基因的重叠启动子
  • DOI:
    10.1038/302800a0
  • 发表时间:
    1983-04-28
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    W. Charles Johnson;Charles P. Moran;Richard Losick
  • 通讯作者:
    Richard Losick

Charles P. Moran的其他文献

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{{ truncateString('Charles P. Moran', 18)}}的其他基金

An Intercellular Connection in Endospore Development
内生孢子发育中的细胞间连接
  • 批准号:
    8669557
  • 财政年份:
    2014
  • 资助金额:
    $ 33.88万
  • 项目类别:
An Intercellular Connection in Endospore Development
内生孢子发育中的细胞间连接
  • 批准号:
    9269236
  • 财政年份:
    2014
  • 资助金额:
    $ 33.88万
  • 项目类别:
An Intercellular Connection in Endospore Development
内生孢子发育中的细胞间连接
  • 批准号:
    8837032
  • 财政年份:
    2014
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA Polymerase Sigma Factors in Streptococcus pyogenes
化脓性链球菌中的 RNA 聚合酶 Sigma 因子
  • 批准号:
    6438077
  • 财政年份:
    2002
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA Polymerase Sigma Factors in Streptococcus pyogenes
化脓性链球菌中的 RNA 聚合酶 Sigma 因子
  • 批准号:
    6699340
  • 财政年份:
    2002
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA Polymerase Sigma Factors in Streptococcus pyogenes
化脓性链球菌中的 RNA 聚合酶 Sigma 因子
  • 批准号:
    6621993
  • 财政年份:
    2002
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA Polymerase Sigma Factors in Streptococcus pyogenes
化脓性链球菌中的 RNA 聚合酶 Sigma 因子
  • 批准号:
    6858581
  • 财政年份:
    2002
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA POLYMERASE AND BACTERIAL DIFFERENTIATION
RNA 聚合酶和细菌分化
  • 批准号:
    6519754
  • 财政年份:
    1996
  • 资助金额:
    $ 33.88万
  • 项目类别:
Bacterial Differentiation
细菌分化
  • 批准号:
    7995244
  • 财政年份:
    1996
  • 资助金额:
    $ 33.88万
  • 项目类别:
RNA POLYMERASE AND BACTERIAL DIFFERENTIATION
RNA 聚合酶和细菌分化
  • 批准号:
    2430502
  • 财政年份:
    1996
  • 资助金额:
    $ 33.88万
  • 项目类别:

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剖析真核转录因子在 DNA 指导的 RNA 聚合酶 II 催化的 RNA 模板转录中的调节作用
  • 批准号:
    10047065
  • 财政年份:
    2020
  • 资助金额:
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  • 项目类别:
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  • 财政年份:
    2015
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