RNA Polymerase Sigma Factors in Streptococcus pyogenes

化脓性链球菌中的 RNA 聚合酶 Sigma 因子

• 批准号: 6621993
• 负责人: Charles P. Moran
• 金额: $ 26.6万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6621993
• 关键词: DNA directed RNA polymerase; Streptococcus pyogenes; bacterial genetics; biological signal transduction; disease /disorder model; gene expression; gene mutation; genetic promoter element; genetic regulation; host organism interaction; laboratory mouse; nuclear runoff assay; site directed mutagenesis; skin infection; transcription factor; virulence

Streptococcus pyogenes (the group A streptococcus or GAS) is an important and common human pathogen. The diseases it causes range from self-limiting skin and throat infections, with, however, the potential for serious sequelae including rheumatic heart disease, acute glomerulonephritis, and possibly pediatric neuropsychological disorders, to severe invasive diseases like myositis and streptococcal toxic shock syndrome. Since single strains of the GAS seem to be able to cause most or all of these diseases, regulation of the expression of GAS genes in response to specific environmental differences within the host is probably key in determining the course of the infectious process. We propose here a new approach to learn more about control of gene expression in the GAS by the characterization of a new RNA polymerase (RNAP) secondary sigma factor needed to transcribe genes under different conditions and the characterization of the genes it regulates.

化脓性链球菌（Streptococcus pyogenes，GAS）是一种重要的人类常见致病菌。 它引起的疾病范围从自限性皮肤和咽喉感染，然而，有可能产生严重的后遗症，包括风湿性心脏病，急性肾小球肾炎，可能还有儿科神经心理障碍，到严重的侵袭性疾病，如肌炎和链球菌中毒性休克综合征。 由于GAS的单一菌株似乎能够引起大多数或所有这些疾病，因此GAS基因表达对宿主内特定环境差异的响应调节可能是决定感染过程的关键。 我们在这里提出了一种新的方法来了解更多关于控制基因表达的气体的表征一种新的RNA聚合酶（RNAP）的第二西格玛因子需要在不同的条件下转录基因和表征的基因，它调节。