MECHANISMS OF NORMAL AND ABNORMAL CRANIOFACIAL MORPHOGEN

正常和异常颅面形态发生机制

• 批准号: 6026936
• 负责人: Jill Helms A Helms
• 金额: $ 7.58万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6026936
• 关键词: antigen antibody reaction; biological signal transduction; chick embryo; congenital disorders; craniofacial; embryo /fetus; embryogenesis; gene expression; histogenesis; laboratory mouse; morphology; oral pharyngeal disorder; quail; retinoids; transcription factor; vertebrate embryology

My career objective is to investigate the genetic control of normal and abnormal craniofacial morphogenesis, My long-term goal is to use this knowledge of the prevention and treatment of craniofacial anomalies. To prepare for this career, I have received clinical training in Dentistry and Periodontology, and basic science training in Developmental Molecular Biology, Embryology and Neurosciences. This diverse educational background has provided me with the necessary scientific foundation to study the complex process of craniofacial development. I believe that the future of craniofacial development lies in understanding how to fully integrate the genetics and embryological processes of brain and head development. Therefore, in order to contribute in a substantial way to this area of research, I propose to expand my clinical training in the areas of Craniofacial Growth and Development, and extend my basic science education to induce advanced instruction in neuroanatomy, Neuroembryology, Biochemistry and mouse genetics. I am an Assistant Professor at UCSF since 1996. I hold a primary appointment in the School of Medicine and a joint appointment in the School of Dentistry. I am a member of two graduate programs, as well as clinical residents and fellows. Currently, I am the advisor to two graduate students and two post-doctoral fellows; I have also acted as the advisor to 8 dental, medical and graduate students as they undertook short-term research projects. My Advisory Committee is comprised of four senior scientists, each of whom is an internationally recognized expert in areas where my training is insufficient. They include Zena Werb, John Robenstein, Caroline Damsky and Karin Vargervik. These individuals have been deeply involved in mentoring me over the last three years. My interactions with these grant proposals (with Drs. Werb and Damsky), and numerous clinical and basic teaching opportunities (with Drs. Vargervik and Damsky). My research proposal focuses on the molecular regulation of craniofacial morphogenesis. We have identified tissues in the facial primordia that act as "organizers", similar to organizers in the limb and brain. Specifically, my research aims are to: 1) characterize the molecular signals that mediate this organizing activity in the face; 2) determine the functional role of these molecules in facial morphogenesis, and 3) gain an understanding of how perturbations in these signals produce the panoply of human craniofacial malformations ranging from cyclopia to facial duplications.

我的职业目标是研究正常和异常颅面形态发生的遗传控制，我的长期目标是利用这些知识预防和治疗颅面异常。为了准备这个职业，我接受了牙科和牙周病学的临床培训，以及发育分子生物学，胚胎学和神经科学的基础科学培训。这种多样化的教育背景为我研究颅面发育的复杂过程提供了必要的科学基础。我相信颅面发育的未来在于理解如何充分整合大脑和头部发育的遗传学和胚胎学过程。因此，为了以实质性的方式为这一研究领域做出贡献，我建议扩大我在颅面生长和发育领域的临床培训，并扩展我的基础科学教育，以诱导神经解剖学，神经胚胎学，生物化学和小鼠遗传学的高级教学。自1996年以来，我一直是UCSF的助理教授。我在医学院担任主要职务，在牙科学院担任联合职务。我是两个研究生项目的成员，也是临床住院医师和研究员。目前，我是两名研究生和两名博士后研究员的顾问;我还担任了8名牙科，医学和研究生的顾问，因为他们进行了短期研究项目。我的咨询委员会由四名资深科学家组成，他们都是国际公认的专家，在我的培训不足的领域。他们包括Zena Werb，John Robenstein，Caroline Damsky和Karin Vargervik。在过去的三年里，这些人一直在深入地指导我。我与这些拨款申请的互动（与Werb博士和Damsky博士），以及许多临床和基础教学机会（与Vargervik博士和Damsky博士）。我的研究计划集中在颅面形态发生的分子调控。我们已经确定了面部原基中充当“组织者”的组织，类似于肢体和大脑中的组织者。具体来说，我的研究目标是：1）表征介导面部组织活动的分子信号; 2）确定这些分子在面部形态发生中的功能作用; 3）了解这些信号的扰动如何产生从独眼畸形到面部重复的人类颅面畸形。