CORE--ANIMAL TISSUE ANALYSIS

组织分析核心将在两个主要领域为与发现综合口腔健康研究中心相关的项目提供技术支持。第一个领域涉及感兴趣的分子的组织制备和定位，包括样品的塑料或石蜡包埋和切片，样品的冷冻和低温切片，定位mRNA的原位杂交，以及定位蛋白质的免疫细胞化学。第二个领域涉及组织提取物或体液的生化测试，包括血液，唾液和沟液。感兴趣的分子包括细胞因子、碳水化合物表位、抗体和尼古丁副产物。预计技术人员将保留所有相同制备程序的记录。预计技术人员将保留所有样品程序的记录。技术人员还将对参与实验室的指定成员进行上述技术培训，以便能够及时完成专业或紧急产品，并使核心实验室及其资源在典型工作日之外得到利用。项目1、2、4、5、6、8、11将是这一核心的主要用户。

The Tissue Analysis Core will provide technical support in two major areas to projects associated with the Comprehensive Oral Health Research Center of Discovery. The first area involves tissue preparation and localization of molecules of interest, including plastic or paraffin embedding and sectioning of samples, freezing and cryostat sectioning of samples, in situ hybridization to localize mRNA, and immunocytochemistry to localize proteins. The second area involve biochemical tests of tissue extracts, or body fluids, including blood, saliva, and crevicular fluid. Molecules of interest include cytokines, carbohydrate epitopes, antibodies, and nicotine byproducts. The technical staff will be expected to keep records of all same preparation procedures. The technical staff will be expected to keep records of all sample procedures. The technical staff will also be expected to train designated members of participating laboratories in the above technologies, so that specialized or rush products can be completed in a timely manner, and so that the core lab and its resources are used beyond the typical workday. Projects 1,2,4,5,6,8,11 will be particularly heavy users of this core.