CORE--ANIMAL TISSUE ANALYSIS

核心——动物组织分析

• 批准号: 6662809
• 负责人: Jill Helms A Helms
• 金额: $ 25.41万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6662809
• 关键词: analytical chemistry; animal tissue; biomedical facility; immunocytochemistry; in situ hybridization; protein localization; tissue /cell preparation

The Tissue Analysis Core will provide technical support in two major areas to projects associated with the Comprehensive Oral Health Research Center of Discovery. The first area involves tissue preparation and localization of molecules of interest, including plastic or paraffin embedding and sectioning of samples, freezing and cryostat sectioning of samples, in situ hybridization to localize mRNA, and immunocytochemistry to localize proteins. The second area involve biochemical tests of tissue extracts, or body fluids, including blood, saliva, and crevicular fluid. Molecules of interest include cytokines, carbohydrate epitopes, antibodies, and nicotine byproducts. The technical staff will be expected to keep records of all same preparation procedures. The technical staff will be expected to keep records of all sample procedures. The technical staff will also be expected to train designated members of participating laboratories in the above technologies, so that specialized or rush products can be completed in a timely manner, and so that the core lab and its resources are used beyond the typical workday. Projects 1,2,4,5,6,8,11 will be particularly heavy users of this core.

组织分析核心将为与综合口腔健康研究中心相关的项目提供两个主要领域的技术支持。第一个领域涉及组织制备和感兴趣分子的定位，包括塑料或石蜡包埋和样品切片、样品冷冻和低温切片、原位杂交定位 mRNA 以及免疫细胞化学定位蛋白质。第二个领域涉及组织提取物或体液（包括血液、唾液和裂隙液）的生化测试。感兴趣的分子包括细胞因子、碳水化合物表位、抗体和尼古丁副产物。技术人员应保留所有相同准备程序的记录。技术人员应保留所有样品程序的记录。技术人员还将对参与实验室的指定成员进行上述技术的培训，以便及时完成专业或紧急产品，并使核心实验室及其资源在典型工作日之外得到利用。项目 1、2、4、5、6、8、11 将是该核心的特别重度用户。