CORE--ANIMAL TISSUE ANALYSIS

组织分析核心将在两个主要领域为与探索综合口腔健康研究中心相关的项目提供技术支持。第一个领域涉及组织制备和感兴趣分子的定位，包括塑料或石蜡包埋和切片，样品的冷冻和冷冻切片，原位杂交定位信使核糖核酸，以及免疫细胞化学定位蛋白质。第二个领域涉及组织提取液或体液的生化测试，包括血液、唾液和阴沟液。感兴趣的分子包括细胞因子、碳水化合物表位、抗体和尼古丁副产物。技术人员将保留所有相同准备程序的记录。预计技术人员将保留所有样本程序的记录。技术人员还将对参与实验室的指定成员进行上述技术培训，以便专业或紧急产品能够及时完成，并使核心实验室及其资源在正常工作日之外得到使用。项目1、2、4、5、6、8、11将是这一核心项目的主要用户。

The Tissue Analysis Core will provide technical support in two major areas to projects associated with the Comprehensive Oral Health Research Center of Discovery. The first area involves tissue preparation and localization of molecules of interest, including plastic or paraffin embedding and sectioning of samples, freezing and cryostat sectioning of samples, in situ hybridization to localize mRNA, and immunocytochemistry to localize proteins. The second area involve biochemical tests of tissue extracts, or body fluids, including blood, saliva, and crevicular fluid. Molecules of interest include cytokines, carbohydrate epitopes, antibodies, and nicotine byproducts. The technical staff will be expected to keep records of all same preparation procedures. The technical staff will be expected to keep records of all sample procedures. The technical staff will also be expected to train designated members of participating laboratories in the above technologies, so that specialized or rush products can be completed in a timely manner, and so that the core lab and its resources are used beyond the typical workday. Projects 1,2,4,5,6,8,11 will be particularly heavy users of this core.