REDUCED LORDOSIS BEHAVIOR AFTER INTRACEREBRAL 8-OH-DPAT

描述(改编自申请人的摘要)： 拟议的研究旨在提供有关这些机制的信息。 5-羟色胺受体相互作用的生理学意义 在控制雌性大鼠的前凸行为方面。重点放在5-羟色胺 在下丘脑内侧基底核，特别是腹内侧核的作用 下丘脑(VMN)。本申请的具体目的是 确定负责这种相互作用的机制。研究还设计了 为了检验5-羟色胺在VMN内的双重功能使 有机体以一种对双方都有利的方式来调整其行为能力 物种和个体的生存。 拟议研究的具体目标是： 测定5-HT1A、5-HT2A和5-HT2C受体的相对分布 在动情前期大鼠和激素刺激的去卵巢大鼠的VMN内 老鼠。 为了测试5-HT1a和5-HT1a作用的细胞内机制 5-HT2A/2C受体。有人提议用实验来检验这一假设 5-HT1a受体介导的抑制cAMP蓄积参与 受体对前凸行为的抑制作用及对cAMP的抑制 蓄积参与了受体对前凸的抑制作用 行为以及5-HT2受体通过作用减弱这种抑制 涉及蛋白激酶C。 有人提议用实验来检验5-HT1a受体减少 去甲肾上腺素(NE)和5-HT2受体的释放促进了这种释放 5-羟色胺对去甲肾上腺素和/或 GABA有助于5-羟色胺抑制和促进前凸的能力 行为。 为了测试5-羟色胺在脑内的双重作用的生理意义。 前凸行为的控制。设计实验是为了评估 假设5-HT2受体减少了对前凸行为的抑制 (通过5-HT1A受体介导)，这是由环境挑战启动的。

DESCRIPTION (Adapted From The Applicant's Abstract): The Proposed studies are designed to provide information about the mechanisms and physiological significance of interactions among serotonin (5-HT) receptors in the control of female rat lordosis behavior. Emphasis is placed on 5-HT's role in the mediobasal hypothalamus (MBH), especially the ventromedial nucleus of the hypothalamus (VMN). The specific aims of the present application are to identify mechanisms responsible for this interaction. Studies are also designed to test the hypothesis that 5-HT's dual function within the VMN enables the organism to modulate its behavioral repertoire in a manner conducive to both species and individual survival. The specific aims of the proposed studies are: To determine the relative distribution of 5-HT1A, 5-HT2A and 5-HT2C receptors within the VMN of proestrous rats and of hormonally-primed, ovariectomized rats. To test intracellular mechanisms responsible for the effects of 5-HT1A and 5-HT2A/2C receptors. Experiments are proposed to test the hypothesis that 5-HT1A receptor-mediated inhibition of cAMP accumulation is involved in the receptor's inhibitory effect on lordosis behavior and that inhibition of cAMP accumulation is involved in the receptor's inibitory effect on lordosis behavior and that 5-HT2 receptors attenuate this inhibition through actions involving protein kinase C. Experiments are proposed to test the hypothesis that 5-HT1A receptors reduce the release of norepinephrine (NE) and that 5-HT2 receptors enhance the release of gamma-aminobutyric acid (GABA); and that these effects of 5-HT on NE and/or GABA contribute to 5-HT's ability to both inhibit and facilitate lordosis behavior. To test a model about the physiological significance of 5-HT's dual role in the control of lordosis behavior. Experiments are designed to evaluate the hypothesis that 5-HT2 receptors reduce inhibition of lordosis behavior (mediated via 5-HT1A receptors) that is initiated by environmental challenge.