IMPROVING UV AND VIOLET SOURCES OF CYTOMETRY

改进细胞分析的紫外线和紫色光源

• 批准号: 6212330
• 负责人: JOHN C WALLING
• 金额: $ 37.38万
• 依托单位: LIGHT AGE, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6212330
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; confocal scanning microscopy; flow cytometry; image enhancement; lasers; ultraviolet microscopy; ultraviolet radiation

The performance of the compact, efficient, highly stable all solid-state ultraviolet laser source created in the Phase I effort , where it was characterized in preliminary cytometry and confocal microscopy evaluation studies, will be further developed to obtain higher power and shorter wavelength. This will permit the source to be of use in a broader class of applications including cytometry that uses shorter wavelength dyes and higher throughput applications such as cell sorting and DNA analysis. A recent extension of this basic laser technology promises to make practical new laser sources that have very short pulses and high repetition rates. This could become an important, if not an enabling, tool for research and for development of commercial instruments that require high peak power for two-photon excitation, such as two-photon microscopy and two-photon 3D microfabrication, and for new optical imaging diagnostics. The proposed Phase II work focuses on applications in cytometry and UV and two-photon microscopy where it is believed that these new laser sources might catalyze the development of new techniques and make these important but expensive diagnostics more widely available. PROPOSED COMMERCIAL APPLICATIONS: It appears likely that such laser sources will be able to replace gas discharge lasers in a host of applications, including laser induced fluorescence, substantially reducing the cost, size and complexity of biotechnology instrumentation that requires intense light at near ultraviolet wavelengths. Significant commercial markets exist for deep red and ultraviolet laser sources that can be gracefully integrated into cytometers and modern microscopes.

在第一阶段工作中创建的紧凑、高效、高度稳定的全固态紫外激光源的性能将进一步发展，以获得更高的功率和更短的波长。这将允许源在更广泛的应用类别中使用，包括使用较短波长染料的细胞术和更高通量的应用，例如细胞分选和DNA分析。这种基本激光技术的最新扩展有望制造出具有非常短脉冲和高重复率的实用新型激光源。这可能成为一个重要的，如果不是一个使能，工具的研究和开发的商业仪器，需要高峰功率的双光子激发，如双光子显微镜和双光子3D微加工，并为新的光学成像诊断。拟议的第二阶段工作侧重于细胞计数和紫外和双光子显微镜的应用，据信这些新的激光源可能会促进新技术的发展，并使这些重要但昂贵的诊断更广泛地应用。拟议的商业应用：这种激光源似乎有可能在许多应用中取代气体放电激光器，包括激光诱导荧光，大大降低了需要近紫外波长强光的生物技术仪器的成本、尺寸和复杂性。深红色和紫外激光源存在着巨大的商业市场，可以优雅地集成到细胞仪和现代显微镜中。