CHARACTERIZATION AND QUANTIFICATION OF IMMOBILIZED DNA

在所提出的工作中，将探讨在金表面上制造有序的寡核苷酸和多核苷酸膜的各种方案。重点将放在自组装单分子膜（SAMs）的形成含有DNA分子或连接到预先形成的链烷硫醇的SAMs的DNA分子。这些薄膜将使用各种表面和分析技术进行量化和表征。本论文将采用五种技术对异质DNA传感器表面进行详细的描述：石英晶体微天平、原子力显微镜、扫描电化学显微镜、伏安法和电化学电感耦合等离子体（ICP）-原子发射光谱法ICP-质谱法。这些技术在DNA固定化、杂交嵌入和膜解吸的研究方面相互补充。我们打算整合的DNA固定化方案的设计，传感表面的表征，以及所得传感器的所得传感器的分析性能。我们的最终目标是了解控制DNA传感器性能的某些重要因素（例如，固定化DNA的表面覆盖率、DNA分子取向、用于分析的探针和靶的最佳浓度，以及开发用于在分子水平上灵敏和选择性地检测核酸的可靠技术）。

In the proposed work, various schemes for fabricating well-ordered oligonucleotide and polynucleotide films onto gold surfaces will be explored. Emphasis will be placed on the formation of self-assembled monolayers (SAMs) containing DNA molecules or attaching DNA molecules onto preformed SAMs of alkanethiols. These films will be quantified and characterized using a variety of surface and analytical techniques. Five techniques will be employed in an effort to provide a detailed description about the heterogeneous DNA sensor surface: quartz crystal microbalance, atomic force microscope, scanning electrochemical microscope, voltammetry, and electrochemical inductively coupled plasma (ICP)-atomic emission spectrometry ICP-mass spectrometry. These techniques complement one another in terms of studies of DNA immobilization, hybridization intercalation, and film desorption. We intend to integrate the design of the DNA immobilization scheme, the characterization of the sensing surface, and the analytical performance of the resulting sensor of the resulting sensor. Our ultimate objective is to understand certain important factors governing DNA sensor performance (e.g., surface coverage of immobilized DNA, DNA molecular orientation, optimal concentrations of the probe and target for an analysis, and the development of an amenable technique for sensitive and selective detection of nucleic acids) at molecular levels.