DIETARY SOY SUPPLEMENTS: CARDIOPROTECTIVE MECHANISMS

本提案的总体目标是确定 (i.e.,特定的大豆黄酮或肽级分）， 对心血管系统的保护作用，评估其 相对生物活性和研究作用机制。以下五 问题将被解决：（1）大豆蛋白的成分是什么， 解释其有益作用或动脉粥样硬化;（2）哪些因素 (i.e.,染料木黄酮或大豆苷元）有助于大豆对 血管功能和动脉粥样硬化;（3）大豆的影响取决于 肽或雌激素的相互作用;（4） 需要特定的雌激素和雌激素来引发和优化这些 影响;（5）雌激素受体依赖性和 大豆在介导心脏保护作用中的独立过程？到 研究大豆中多种成分的单独和组合作用， 动脉粥样硬化斑块的发展，这在人类中不易研究 受试者和非人灵长类动物，具有诱导基因突变的小鼠， 动脉粥样硬化的易感性增加和/或 将使用雌激素受体缺乏的药物。拟议的研究将 评估大豆的各个成分的作用及其可能的 在介导主要心脏保护作用中的相互作用， 据报道，大豆。这一结果将进一步促进对大豆的了解。 心脏保护特性，并导致优化其有利效果的策略。

The overall objective of this proposal is to identify the component(s) (i.e., specific isoflavones or peptide fractions) of soy that account for its protective effects on the cardiovascular system, assess their relative bioactivity and study mechanisms of action. The five following questions will be addressed: (1) what are the constituents of soy protein that account for its beneficial effects or atherogenesis; (2) which isoflavones (i.e., genistein or daidzein) contribute to soy's favorable effects on vasomotor function and atherosclerosis; (3) do the effects of soy depend on isoflavone peptide or isoflavone estrogen interactions; (4) what amounts of specific isoflavones and estrogen are needed to elicit and optimize these effects; and (5) what are the relative roles of estrogen receptor dependent and independent processes in mediating the cardioprotective effects of soy? To study the separate and combined effects of many components of soy on the development of atherosclerotic plaques, which cannot be studied easily in human subjects and nonhuman primates, mice with induced genetic mutations that result in increased susceptibility to the development of atherosclerosis and/or a deficiency in estrogen receptors will be used. The studies proposed will evaluate the role of the individual constituents of soy and their possible interactive effects in mediating the major cardioprotective effects that have been reported for soy. The results will further advance understanding of soy's cardioprotective properties and lead to strategies for optimizing its favorable effects.