REGULATION OF SEROTONERGIC NEUROTRANSMISSION

描述（改编自申请人摘要）： 5-羟色胺是一种神经递质和神经调质， 脊椎动物和无脊椎动物的攻击、运动和肠道运动。在 在过去的几年里，人们发现血清素是“借来的”，而不是 由脊椎动物的一些中枢和外周神经元合成。最近 结果表明，一组60个神经元，支配后肠的， 小龙虾利用血清素作为神经递质。而不是合成 然而，这些后肠神经元（HGN）似乎“借用”了血清素， 从外部来源获取信息。这些吸收机制似乎是 受外感受性和本体感受性感觉输入的控制。的 本研究的总体目标是研究 这种“借来的发射器”现象在craylish，并确定它是如何 有助于控制螯虾中的多巴胺能调节。 具体而言，拟议的研究将定量确定是否 5-羟色胺优先被HGN借用或合成。的机制 包括那些控制血清素供应的 由外部来源在HGN附近提供的，以及 控制HGN的5-羟色胺摄取和释放。使用 将确定HGN对血清素的影响， 在其他邻近的和研究充分的多巴胺能系统上的血清素摄取， 包括那些调整姿势和逃跑的人。

DESCRIPTION(Adapted from applicant's abstract): Serotonin is a neurotransmitter and neuromodulator used in the control of aggression, movement, and gut motility in vertebrates and invertebrates. In the past few years, serotonin has been found to be "borrowed" rather than synthesized by some central and peripheral neurons in vertebrates. Recent results indicate that a set of 60 neurons that innervate the hindgut of crayfish employ serotonin as a neurotransmitter. Instead of synthesizing serotonin, however, these hindgut neurons (HGNs) appear to "borrow" serotonin by taking it up from external sources. These uptake mechanisms appear to be under the control of exteroceptive and proprioceptive sensory input. The overall goal of this research is to investigate the mechanisms that underlie this "borrowed transmitter" phenomenon in craylish, and to determine how it contributes to the control of serotonergic modulation in the crayfish. Specifically, the proposed research will determine quantitatively whether serotonin is preferentially borrowed or synthesized by the HGNs. The mechanisms that govern borrowing, including those that controi the supply of serotonin made available in the vicinity of the HGNs by external sources, and those that control serotonin uptake and release by the HGNs, will be investigated. The use to which serotonin is put by the HGNs will be determined, as will the effects of serotonin uptake on other adjacent and well-studied serotonergic systems, including those that modulate posture and escape.