ENZYMOLOGY

酶学

• 批准号: 6346010
• 负责人: RAYMOND J BUDDE
• 金额: $ 14.64万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346010
• 关键词: antineoplastics; combinatorial chemistry; cooperative study; drug design /synthesis /production; enzyme activity; enzyme inhibitors; molecular cloning; protein tyrosine kinase

This Laboratory Program represents the biochemistry section of a multidisciplinary effort to develop specific inhibitors against Src. This Program will be responsible for producing recombinant Src and other PTKs to (1) screen inhibitors, (2) determine the affinity of "hits" identified in the screen, (3) determine the specificity of the "hits" identified in the screen, and (4) perform structural studies of the Src active site. This laboratory program serves as a screen between the two synthetic chemistry laboratories and the two biological testing laboratories which functions to narrow down the thousands of compounds synthesized to those which are worth studying for biological efficacy. In addiction, it aids the synthetic chemistry sections in choosing which analogs to consider for synthesis and testing. The biochemistry lab has developed an infrastructure to produce recombinant PTKs and a high-through put screening of combinatorial libraries.

本实验室程序代表a的生物化学部分