CONTINUOUSLY ENERGY DISPERSIVE XRAY SCATTERING

连续能量色散 X 射线散射

• 批准号: 6339358
• 负责人: Zhong Ren
• 金额: $ 2.91万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6339358
• 关键词: biomedical resource; structural biology

The continuous energy-dispersive nature of the Laue method is perhaps the most unique feature among all diffraction methods. We propose to extract the anomalous scattering signal from an entire Laue data set at every energy in the continuous incident X ray spectrum. We will design a new phasing scheme and its software implementation for macromolecular structure determination based on the energy-dispersive anomalous scattering signal from one and only one Laue data set. We will compare this scheme to the multiwavelength anomalous diffraction (MAD) method, a proven successful phasing scheme based on monochromatic oscillation data sets obtained at several specific, monochromatic wavelengths around the absorption edge of a heavy element. The new scheme will not only provide structural biologists another phasing tool but also greatly improve the speed to collect sufficient data in order to phase a macromolecular structure. I I

劳厄方法的连续能量色散性质是 也许是所有衍射方法中最独特的特征。 我们 提出从整个 Laue 中提取异常散射信号 连续入射 X 射线光谱中每个能量的数据集。 我们将设计一个新的分阶段方案及其软件实现 用于大分子结构测定 来自一个且唯一一个的能量色散异常散射信号 劳厄数据集。 我们将该方案与多波长方案进行比较 反常衍射 (MAD) 方法，一种经过验证的成功定相方案 基于在多个位置获得的单色振荡数据集 吸收边缘附近的特定单色波长 重元素。 新计划不仅会提供结构性的 生物学家的另一种定相工具，而且还大大提高了速度 收集足够的数据以定相大分子结构。 我我