MOLECULAR EVOLUTIONARY GENETICS OF COLOR VISION

色觉的分子进化遗传学

• 批准号: 6180738
• 负责人: WEN-HSIUNG LI
• 金额: $ 16.84万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180738
• 关键词: Aotus; Carnivora; Chiroptera; Marsupialia; Prosimii; Rodentias; biochemical evolution; bioperiodicity; color visions; gene expression; genetic polymorphism; molecular cloning; nucleic acid sequence; opossums; polymerase chain reaction; protein structure function; rhodopsin; statistics /biometry; visual pigments

DESCRIPTION: (Adapted from investigator's abstract) The goal of this 2-year project is to understand the effects of nocturnal life on the function and evolution of the short and middle-to-long wavelength opsin genes and to know whether the opsin sequences of a mammal can help infer the life style (diurnal or nocturnal) of its ancestor. The specific issues are: 1) To test the hypothesis that there was a loss-and-regain of the S and M/L opsins in the ancestral eutherians. 2) To know how fast loss of the S opsin gene can occur under nocturnal life. 3) Has the M/L opsin gene always persisted in all (nocturnal) mammals? 4) Is the M/L opsin locus polymorphic in any (especially nocturnal) prosimians as in the case for New World monkeys? And is it polymorphic in owl monkeys (the only nocturnal New World monkey? 5)Did the common ancestor of higher primates have an M or L opsin? 6) To examine the assumption of long strong nocturnality in several animals. To examine these issues, the PI proposes the following work: 1) Sequence the M/L and S opsin genes in representative individuals from a series of species: Two marsupials (opossum and red-necked wallaby); One monotreme (platypus); One owl monkey and 8 prosimians; Four bats (two mega, two micro); Three rodents; Three carnivores; Study whether there is M/L polymorphism in: Tarsiers; Lemurs; Owl monkeys.

描述：（改编自研究者摘要）本2年研究的目标 项目是了解夜间生活对功能的影响， 短波长和中长波长视蛋白基因的进化， 哺乳动物的视蛋白序列是否可以帮助推断生活方式（昼夜 或夜，其祖先。具体问题是：1）测试 假设有一个损失和恢复的S和M/L视蛋白在 祖先真兽目2)为了了解S视蛋白基因丢失的速度， 在夜间生活。3)M/L视蛋白基因是否一直存在于所有 （夜间）哺乳动物？4)M/L视蛋白基因座在任何（特别是） 夜行性的原猴纲动物，就像新大陆的猴子一样？是不是 猫头鹰猴（唯一的夜间活动的新大陆猴？5)做了 高等灵长类动物的共同祖先有M或L视蛋白？6)审查 假设在几种动物中存在长时间的强非线性。 为了研究这些问题，PI建议开展以下工作：1） 来自一系列物种的代表性个体中的M/L和S视蛋白基因： 两个有袋动物（负鼠和红颈袋鼠）;一个单孔目动物（鸭嘴兽）;一个 猫头鹰猴和8个原猴; 4个蝙蝠（2个巨型，2个微型）; 3个啮齿动物; 三种食肉动物;研究眼镜猴、狐猴、枭猴是否存在M/L多态性。

项目成果

Identification of essential amino acid changes in paired domain evolution using a novel combination of evolutionary analysis and in vitro and in vivo studies.

• DOI: 10.1093/oxfordjournals.molbev.a004212
• 发表时间: 2002-09
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Hongmin Sun;S. Merugu;X. Gu;Y. Kang;D. P. Dickinson;P. Callaerts;Wen-Hsiung Li