FULL-LENGTH MRNA AND CDNA METHODOLOGY

全长 mRNA 和 CDNA 方法学

• 批准号: 6175361
• 负责人: WILLIAM A RIEDL
• 金额: $ 13.64万
• 依托单位: EXPRESSED GENOMICS, LLC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2003-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6175361
• 关键词: animal genetic material tag; animal tissue; chimeric proteins; complementary DNA; eukaryote; genetic library; human genetic material tag; human tissue; laboratory mouse; messenger RNA; molecular site; northern blottings; nucleic acid hybridization; nucleic acid sequence; posttranscriptional RNA processing; protein purification; surface plasmon resonance; technology /technique development; transfection /expression vector; translation factor

Our goal is to apply the affinity of eIF4E to the 5' cap structure of mRNA to the purification of Full-Length mRNA, 5' enriched mRNA, and cDNA mRNA first strand products. This technology help fill the current full-length sequence gaps in cDNA databases. The cDNA library construction will preserve the full-length nature of the full-length mRNA.

我们的目标是将eIF4E对mRNA的5'帽结构的亲和力应用于全长mRNA、5'富集mRNA和cDNA mRNA第一链产物的纯化。 这项技术有助于填补cDNA数据库中目前的全长序列空白。 cDNA文库构建将保留全长mRNA的全长性质。