FULL-LENGTH MRNA AND CDNA METHODOLOGY
FULL-LENGTH MRNA AND CDNA METHODOLOGY
批准号:
6175361
负责人:
WILLIAM A RIEDL
金额:
$13.64万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-09-30 至 2003-09-30
关键词:
animal genetic material tag animal tissue chimeric proteins complementary DNA eukaryote genetic library human genetic material tag human tissue laboratory mouse messenger RNA molecular site northern blottings nucleic acid hybridization nucleic acid sequence posttranscriptional RNA processing protein purification surface plasmon resonance technology /technique development transfection /expression vector translation factor
中文摘要
我们的目标是将eIF4E对mRNA的5'帽结构的亲和力应用于全长mRNA、5'富集mRNA和cDNA mRNA第一链产物的纯化。 这项技术有助于填补cDNA数据库中目前的全长序列空白。 cDNA文库构建将保留全长mRNA的全长性质。
英文摘要
Our goal is to apply the affinity of eIF4E to the 5' cap structure of mRNA to the purification of Full-Length mRNA, 5' enriched mRNA, and cDNA mRNA first strand products. This technology help fill the current full-length sequence gaps in cDNA databases. The cDNA library construction will preserve the full-length nature of the full-length mRNA.
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FULL LENGTH MRNA AND CDNA METHODOLOGY
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批准号:6080856
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项目类别:
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资助金额:$13.35万
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财政年份:1999
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负责人:WILLIAM A RIEDL
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依托单位:
海外基金